一株支原体质粒的分离及限制性核酸内切酶分析

Isolation and restriction endonuclease analysis of a mycoplasma plasmid.

作者信息

Bergemann A D, Finch L R

机构信息

Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.

出版信息

Plasmid. 1988 Jan;19(1):68-70. doi: 10.1016/0147-619x(88)90064-9.

DOI:10.1016/0147-619x(88)90064-9

PMID:2840682

Abstract

A 1.7-kb plasmid was isolated from a large colony type strain of Mycoplasma mycoides subsp. mycoides. It was cloned into the Escherichia coli vector M13mp19, and DNA from the resultant recombinant was used to construct a restriction map of the plasmid. Because mycoplasmas show deviation from normal coding patterns, the availability of a mycoplasma plasmid could be useful in the development of a cloning vector for them.

摘要

从蕈状支原体丝状亚种的一个大菌落型菌株中分离出一个1.7千碱基对的质粒。它被克隆到大肠杆菌载体M13mp19中，所得重组体的DNA被用于构建该质粒的限制性图谱。由于支原体表现出与正常编码模式的偏差，支原体质粒的可用性可能有助于为它们开发克隆载体。