King K W, Dybvig K
Department of Comparative Medicine, University of Alabama at Birmingham 35294.
Plasmid. 1994 May;31(3):308-11. doi: 10.1006/plas.1994.1033.
Plasmids pIK delta and pIK delta-erm have recently been developed as mycoplasmal cloning vectors. In this report, we demonstrate that these plasmids can replicate in Mycoplasma capricolum, a mycoplasmal species for which transformation had not previously been characterized. Both plasmids are stably maintained at a higher copy number than in their parental species, Mycoplasma mycoides subsp. mycoides. We have also examined the possibility of one or more restriction-modification systems affecting transformation frequencies in both species.
质粒pIK delta和pIK delta-erm最近被开发为支原体克隆载体。在本报告中，我们证明这些质粒能够在山羊支原体中复制，山羊支原体是一种之前未对其转化特性进行过描述的支原体物种。这两种质粒在山羊支原体中均能稳定维持，且拷贝数高于其亲本物种——丝状支原体丝状亚种。我们还研究了一个或多个限制修饰系统影响这两个物种转化频率的可能性。
