小鼠亚急性苯中毒时肝脏的组织化学和组织酶学变化

Histochemical and histoenzymatic changes in mouse liver in subacute benzene intoxication.

作者信息

Kamiński M, Jonek J J, Konecki J, Kamińska O, Gruszeczka B, Koehler B

出版信息

Acta Histochem. 1978;61(1):1-19. doi: 10.1016/S0065-1281(78)80043-9.

PMID:209652

Abstract

The investigations were performed on mice. They were divided into a control group and 4 experimental groups. The experimental animals were administered intraperitoneally benzene 6 X every 24 h. The animals were decapitated 30 min. 4, 12 and 24 h after the last benzene administration. During the experiment, dyeing for neutral lipids and glycogen was carried out, and the activity of NADH2-r.t., SDH, G-6-Pase, ATP-ase and ACP was estimated. A decrease of glycogen content in liver cells, deviations in the amount of neutral lipids, reversible decrease of mitochondrial enzymes activity, and intensification of the processes of intracellular catabolism were found.

摘要

这些研究是在小鼠身上进行的。它们被分为一个对照组和4个实验组。实验动物每24小时腹腔注射苯6次。在最后一次注射苯后30分钟、4小时、12小时和24小时将动物断头。在实验过程中，对中性脂质和糖原进行染色，并评估NADH2-r.t.、SDH、G-6-Pase、ATP-ase和ACP的活性。发现肝细胞中糖原含量降低、中性脂质数量出现偏差、线粒体酶活性可逆性降低以及细胞内分解代谢过程增强。