Institute for Fetal Origin Diseases, First Hospital of Soochow University & Prenatal Biology, Center of Soochow University, Suzhou 215006, China.
Peptides. 2011 Jan;32(1):104-11. doi: 10.1016/j.peptides.2010.10.012. Epub 2010 Oct 19.
The present study determined water and salt intake as well as expression of AT(1) and AT(2) receptors in the brain and kidney in the adult offspring rats prenatally exposed to high sucrose. Following the exposure during pregnancy, water intake and salt intake at baseline levels were not changed in the adult offspring. However, after 24h water deprivation, consumption of water and salt was significantly increased compared to that of the control. Plasma sodium and osmolality levels remained the same between the offspring in the control and the exposed groups, while hematocrit was higher in the offspring exposed to prenatal high sucrose immediately following water deprivation. Density of renal AT(1) receptor protein was the same between the control and the exposed group, while AT(2) receptor protein in the kidney was significantly increased in the offspring exposed to prenatal high sucrose in association of thicker basal membrane of glomerular. In the forebrain, both AT(1) and AT(2) receptor levels were significantly increased in the offspring with history of prenatal high sucrose. In addition, water deprivation induced more c-fos expression in the central dipsogenic areas, including the paraventricular and supraoptic nuclei in the offspring exposed to prenatal high sucrose. The results suggested that prenatal high intake of sucrose may affect development of pathways in regulation of dipsogenic behavior in face of dehydration, which was associated with altered expression of AT(1) or/and AT(2) receptors in the kidney and brain.
本研究旨在探讨产前高蔗糖暴露对成年子代大鼠水盐摄入、脑和肾脏中 AT(1)和 AT(2)受体表达的影响。在孕期暴露后，成年子代大鼠的基础水平水和盐摄入没有改变。然而，在 24 小时断水后，与对照组相比，其水和盐的消耗量显著增加。对照组和暴露组的子代血浆钠和渗透压水平相同，而在断水后立即，暴露于产前高蔗糖的子代的红细胞压积更高。肾脏 AT(1)受体蛋白密度在对照组和暴露组之间相同，而暴露于产前高蔗糖的子代肾脏中的 AT(2)受体蛋白显著增加，同时肾小球基底膜增厚。在前脑，有产前高蔗糖史的子代的 AT(1)和 AT(2)受体水平均显著升高。此外，在暴露于产前高蔗糖的子代中，水剥夺诱导了更多的 c-fos 在中枢性口渴区域的表达，包括室旁核和视上核。结果表明，产前高蔗糖摄入可能会影响面对脱水时口渴行为调节途径的发育，这与肾脏和大脑中 AT(1)或/和 AT(2)受体表达的改变有关。
