Phytopharmacology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
J Ethnopharmacol. 2011 Jan 27;133(2):480-3. doi: 10.1016/j.jep.2010.10.025. Epub 2010 Oct 19.
Nervilia plicata (Orchidaceae) has long been used in the antidiabetic medicinal preparations of traditional healers of Wayanad (Kerala), but recuperative potential of the plant was remained undefined. We demonstrated the regenerative potential of the plant extract on kidney affected by type 2 diabetes besides lowering blood glucose.
The aim of the current study was to investigate the recuperative and regenerative potential of alcoholic stem extract of Nervilia plicata on streptozotocin-nicotinamide induced type 2 diabetic models.
Non insulin dependent diabetes mellitus (NIDDM) was induced in overnight fasted rats by intramuscular injection (IMI) of 60 mg/kg STZ and 120 mg/kg of nicotinamide after 5 min interval. Blood glucose was assessed by a glucometer, serum urea and creatinine levels were determined by diacetylmonooxime method and Jaffe reaction respectively. Kidney sections were taken and stained with Masson's tri-dye and Periodic Acid Schiff (PAS) and examined for structural changes. Also lipid peroxidation product (LPP) levels were determined as thio barbituric acid reactive substance levels (TBARS) method.
On administration of 5 mg/kg of plant extract, blood glucose levels of the NIDDM rats showed 62.00 and 76.29% decrease in the blood glucose levels on day 0 and day 30 respectively. Damages caused to the kidney tissue were negligible or not seen. Serum urea and creatinine levels showed 61.49 and 70.96% decrease on day 30. LPP levels of kidney and pancreas showed 70.58 and 77.41% decrease respectively.
These results demonstrate significant antidiabetic and regenerative potential of the Nervilia plicata, justifying the use of plant in the indigenous system of medicine. Isolation and characterisation of the compound(s) playing pivotal role in the cure would open new vistas in the therapy of type 2 diabetes.
细距石豆兰（兰科）长期以来一直被用于喀拉拉邦韦丹纳德（Wayanad）传统治疗师的抗糖尿病药物制剂中，但该植物的恢复潜力仍未得到明确界定。我们证明了植物提取物在降低血糖的同时对 2 型糖尿病影响的肾脏具有再生潜力。
本研究的目的是研究细距石豆兰的酒精茎提取物对链脲佐菌素-烟酰胺诱导的 2 型糖尿病模型的恢复和再生潜力。
通过 5 分钟间隔的肌肉内注射（IMI）60mg/kg STZ 和 120mg/kg 烟酰胺，使 overnight 禁食大鼠产生非胰岛素依赖型糖尿病（NIDDM）。通过血糖仪评估血糖，通过二乙酰一肟法和 Jaffe 反应分别测定血清尿素和肌酐水平。取肾组织切片，用 Masson 三色染料和过碘酸希夫（PAS）染色，观察结构变化。还通过硫代巴比妥酸反应物质（TBARS）法测定脂质过氧化产物（LPP）水平。
在给予 5mg/kg 植物提取物时，NIDDM 大鼠的血糖水平在第 0 天和第 30 天分别降低了 62.00%和 76.29%。对肾脏组织造成的损害可以忽略不计或未观察到。第 30 天血清尿素和肌酐水平分别降低了 61.49%和 70.96%。肾脏和胰腺的 LPP 水平分别降低了 70.58%和 77.41%。
这些结果表明细距石豆兰具有显著的抗糖尿病和再生潜力，证明了该植物在本土医学中的应用。分离和鉴定在治疗中起关键作用的化合物将为 2 型糖尿病的治疗开辟新的前景。
