Farmacognosia, Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba (IMBIV-CONICET), Ciudad Universitaria, Córdoba, Argentina.
J Photochem Photobiol B. 2011 Feb 7;102(2):108-14. doi: 10.1016/j.jphotobiol.2010.09.009. Epub 2010 Sep 29.
Photosensitizing anthraquinones isolated from Heterophyllaea pustulata Hook f. (Rubiaceae), namely soranjidiol, rubiadin, damnacanthal and 5,5'-bisoranjidiol, showed antibacterial activity (bacteriostatic/bactericide) on Staphylococcus aureus. The mechanism of action seems to involve an increase in the levels of superoxide anion (O(2)(·-)) and/or singlet molecular oxygen ((1)O(2)). Moreover, the effect of actinic irradiation as a boosting agent for the production of both reactive species of oxygen as well as its influence on antibacterial activity was assessed. The routine susceptibility assay (minimum inhibitory concentration determination) was carried out by means of the broth macrodilution method. Bactericide activity was determined counting the colony-forming units per milliliter (cfu/mL) in plate. The O(2)(·-) production was determined by means of an indirect photobiological assay (Nitroblue Tetrazolium test), and the production of (1)O(2) was followed using an indirect steady-state method, with methionine as the (1)O(2) chemical quencher.
从 Heterophyllaea pustulata Hook f.（茜草科）中分离出的光敏蒽醌，即soranjidiol、rubiadin、damnacanthal 和 5,5'-bisoranjidiol，对金黄色葡萄球菌具有抗菌活性（抑菌/杀菌）。作用机制似乎涉及超氧阴离子 (O(2)(·-)) 和/或单线态氧 ((1)O(2)) 水平的增加。此外，还评估了光致发光照射作为产生两种活性氧物质的增强剂及其对抗菌活性的影响。通过肉汤微量稀释法进行常规药敏试验（最小抑菌浓度测定）。通过平板计数每毫升形成的菌落形成单位 (cfu/mL) 来确定杀菌活性。通过间接光生物测定法（硝基四唑蓝试验）测定 O(2)(·-) 的产生，并用间接稳态法，以蛋氨酸作为 (1)O(2) 的化学猝灭剂来跟踪 (1)O(2) 的产生。
