Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Ciencias Farmacéuticas, Córdoba, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto Multidisciplinario de Biología Vegetal (IMBIV), Córdoba, Argentina.
Planta Med. 2021 Aug;87(9):716-723. doi: 10.1055/a-1345-6831. Epub 2021 Feb 23.
is a phototoxic plant from Argentina. Aerial parts extracts, high in photosensitizing anthraquinones, have shown antiviral activity. The purpose of this study was to study the antiherpetic activity of the main purified anthraquinones, even evaluating their competence as photodynamic sensitizers to photo-stimulate the antiviral effect. antiviral activity against Herpes Simplex virus type I and the photo-inactivation of viral particle were studied by the Neutral Red uptake test and observation of the cytopathic effect. Rubiadin 1-methyl ether and 5,5'-bisoranjidiol produced a significant effect (≥ 80% inhibition) with minimal damage to host cells (subtoxic concentration). Anthraquinones with poor antiherpetic activity at its maximum noncytotoxic concentration showed an important photo-stimulated effect, such is the case of soranjidiol and 5,5'-bisoranjidiol (28.0 ± 6.3 81.8 ± 2.1% and 15.5 ± 0.3 89.8 ± 1.7%, respectively). The study also proved the decrease of viral particles, necessary to reduce infection. Therefore, photosensitizing anthraquinones from natural resources could be proposed to develop new treatments for localized viral lesions with antimicrobial photodynamic therapy.
是一种来自阿根廷的光毒性植物。高含量具有光敏作用的蒽醌类化合物的地上部分提取物具有抗病毒活性。本研究的目的是研究主要纯化蒽醌类化合物的抗疱疹活性，甚至评估它们作为光动力敏化剂的能力，以光刺激抗病毒作用。通过中性红摄取试验和观察细胞病变效应，研究了抗单纯疱疹病毒 I 型的活性和病毒粒子的光失活作用。红紫素 1-甲醚和 5,5'-双橙皮苷二氢查耳酮产生了显著的效果（≥80%抑制），同时对宿主细胞的损伤最小（亚毒性浓度）。在最大非细胞毒性浓度下抗疱疹活性差的蒽醌类化合物表现出重要的光刺激作用，例如橙皮苷二氢查耳酮和 5,5'-双橙皮苷二氢查耳酮（分别为 28.0±6.3%和 81.8±2.1%以及 15.5±0.3%和 89.8±1.7%）。该研究还证明了减少病毒颗粒的必要性，以减少感染。因此，从天然资源中提取的光敏化蒽醌类化合物可以被提议用于开发新的治疗方法，以对抗局部病毒病变的抗菌光动力疗法。
