Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran; Biotechnology Center, Research Institute of Petroleum Industry, Tehran, Iran.
Immunol Lett. 2009 Sep 22;126(1-2):48-53. doi: 10.1016/j.imlet.2009.07.012. Epub 2009 Aug 5.
Tuberculosis (TB) represents one of the leading killers among all infectious disease. Protection against TB depends on the activation of T-helper type I (Th1) immune response. Carbon nanotubes (CNTs) have attracted considerable attention because of their potential applications as new nanovehicle. In the current study, tuberculin purified protein derivative (PPD) was conjugated to carboxylated single-walled carbon nanotubes (SWCNTs). Cytotoxicity of the carboxylated SWCNT and SWCNT-PPD conjugate was analyzed with MTT assay and by reactive oxygen species (ROS) and nitric oxide (NO) generation. Male BALB/c mice were immunized with BCG, PPD, SWCNT-PPD conjugate and PPD in complete Freund's adjuvant (CFA). Induction of cellular immune response was analyzed by measuring the levels of Th1 cytokines (IFN-gamma and IL-12) and Th2 cytokines (IL-10 and IL-5). Immunization with non-conjugated PPD or PPD in Freund's adjuvant induced a Th2 cytokine response while immunization with BCG resulted to a mixed Th1/Th2 cytokine response. In contrast, PPD in conjugation with SWCNT generated preferentially a Th1-type cytokine response in the absence of potential cytotoxic effects.
结核病（TB）是所有传染病中主要的致死原因之一。预防结核病依赖于 T 辅助型 1（Th1）免疫反应的激活。由于碳纳米管（CNTs）在作为新型纳米载体方面的潜在应用，它们引起了相当大的关注。在本研究中，结核菌素纯蛋白衍生物（PPD）被连接到羧基化单壁碳纳米管（SWCNTs）上。通过 MTT 分析和活性氧（ROS）和一氧化氮（NO）生成来分析羧基化 SWCNT 和 SWCNT-PPD 缀合物的细胞毒性。雄性 BALB/c 小鼠用卡介苗（BCG）、PPD、SWCNT-PPD 缀合物和 PPD 在完全弗氏佐剂（CFA）中免疫。通过测量 Th1 细胞因子（IFN-γ和 IL-12）和 Th2 细胞因子（IL-10 和 IL-5）的水平来分析细胞免疫反应。与未缀合的 PPD 或 PPD 在 Freund 佐剂中的免疫诱导 Th2 细胞因子反应，而 BCG 的免疫导致混合的 Th1/Th2 细胞因子反应。相比之下，PPD 与 SWCNT 的缀合在没有潜在细胞毒性作用的情况下优先产生 Th1 型细胞因子反应。
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