Program in Chemical Biology, Department of Physiology and Pharmacology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA.
Curr Opin Chem Biol. 2010 Dec;14(6):797-802. doi: 10.1016/j.cbpa.2010.09.014. Epub 2010 Oct 19.
Expressed protein ligation (EPL) is a semisynthetic technique for the chemoselective ligation of a synthetic peptide to a recombinant peptide that results in a native peptide bond at the ligation site. EPL therefore allows us to engineer proteins with chemically defined, site-specific modifications. While EPL has been used mainly in investigations of soluble proteins, in recent years it has been increasingly used in investigations of integral membrane proteins. These include studies on the KcsA K(+) channel, the non-selective cation channel NaK, and the porin OmpF. These studies are discussed in this review.
表达蛋白连接（EPL）是一种半合成技术，用于在连接位点处化学选择性地连接合成肽和重组肽，从而形成天然肽键。因此，EPL 允许我们用化学定义的、定点修饰的方法来设计蛋白质。虽然 EPL 主要用于可溶性蛋白的研究，但近年来，它越来越多地用于研究整合膜蛋白。这些研究包括对 KcsA K(+)通道、非选择性阳离子通道 NaK 和孔蛋白 OmpF 的研究。本综述讨论了这些研究。
