Tada Seiichi, Kitajima Takashi, Ito Yoshihiro
Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Int J Mol Sci. 2012;13(5):6053-6072. doi: 10.3390/ijms13056053. Epub 2012 May 18.
Growth factors play important roles in tissue regeneration. However, because of their instability and diffusible nature, improvements in their performance would be desirable for therapeutic applications. Conferring binding affinities would be one way to improve their applicability. Here we review techniques for conjugating growth factors to polypeptides with particular affinities. Conjugation has been designed at the level of gene fusion and of polypeptide ligation. We summarize and discuss the designs and applications of binding growth factors prepared by such conjugation approaches.
生长因子在组织再生中发挥着重要作用。然而，由于其不稳定性和可扩散性，为了治疗应用，需要改进它们的性能。赋予结合亲和力将是提高其适用性的一种方法。在这里，我们综述了将生长因子与具有特定亲和力的多肽偶联的技术。偶联已在基因融合和多肽连接水平上进行了设计。我们总结并讨论了通过这种偶联方法制备的结合生长因子的设计和应用。
