Liu Hui-mi, Zhao Hong
Department of Neurology, Fourth Affiliated Hospital of Haerbin Medical University, Haerbin 150001, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Oct;30(10):2380-3.
To compare the effect of botulinum neurotoxin A (BTA) and denervation in inducing masseter muscle atrophy in rabbits to provide experimental evidence for the safe clinical application of BTA.
Seventy-five healthy adult New Zealand white rabbits were randomized equally into denervation group, BTA group and normal control group. In the former two groups, the rabbits were subjected to right masseter muscle denervation and botulinum (3 µg/kg) injection at the muscle, respectively, with the left side as the control. The thickness of the denervated masseter was determined using B type ultrasound and its weight measured after the treatment.
The thickness of the masseter muscle on the experimental side was significantly decreased to 50.80% and 54.07%, and its weight to 66.80% and 56.16% of the normal level after denervation and BTA injection, respectively.
BTA-induced denervation produces less potent atrophy-inducing effect on skeletal muscle than surgical denervation. BTA causes atrophy mainly at the injection site of the target muscle without significant diffusion or toxicity to the muscular cells.
比较A型肉毒杆菌神经毒素（BTA）和去神经支配对兔咬肌萎缩的诱导作用，为BTA临床安全应用提供实验依据。
将75只健康成年新西兰白兔随机均分为去神经支配组、BTA组和正常对照组。前两组分别对兔右侧咬肌进行去神经支配和肌肉注射肉毒杆菌（3μg/kg），以左侧作为对照。采用B型超声测定去神经支配咬肌的厚度，并在治疗后测量其重量。
去神经支配和注射BTA后，实验侧咬肌厚度分别显著降至正常水平的50.80%和54.07%，重量分别降至正常水平的66.80%和56.16%。
BTA诱导的去神经支配对骨骼肌的萎缩诱导作用比手术去神经支配弱。BTA主要在靶肌肉注射部位引起萎缩，对肌细胞无明显扩散或毒性作用。
