Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia 6009, Australia.
J Mol Histol. 2010 Dec;41(6):309-14. doi: 10.1007/s10735-010-9287-1. Epub 2010 Oct 23.
Epidermal Growth Factor (EGF) has been identified as playing a critical role in the wound healing process. The objective of this study is to investigate the role that EGF plays in rat tympanic membrane (TM) wound healing using two techniques, microarray and immunohistochemistry. The tympanic membranes of rats were perforated using a sterile needle and sacrificed at time points during 2 weeks following perforation. The normalized signal intensities at the time points for EGF and associated genes are presented. The rat EGF mRNA did not change significantly between time points. Five associated proteins, including heparin-binding EGF-like growth factor were found to be differentially expressed above a two fold threshold at 12 h following perforation. EGF staining was found at low levels in the uninjured TM. Levels of EGF staining increased at 24 h in the basal keratinocyte layer, became diffusely elevated in the specimen at 36 h, before a second peak in staining of the keratinocyte layer at Day 4. The staining of EGF corresponds to its multiple roles in TM wound healing.
表皮生长因子（EGF）已被确定在伤口愈合过程中发挥关键作用。本研究旨在使用两种技术，即微阵列和免疫组织化学，研究 EGF 在大鼠鼓膜（TM）创伤愈合中的作用。使用无菌针在大鼠的鼓膜上穿孔，并在穿孔后 2 周的时间点处死。呈现 EGF 和相关基因在时间点的归一化信号强度。大鼠 EGF mRNA 在时间点之间没有显著变化。发现五种相关蛋白，包括肝素结合 EGF 样生长因子，在穿孔后 12 小时的两倍以上阈值处表现出差异表达。在未受伤的 TM 中，EGF 染色水平较低。在 24 小时时，在基底角质形成细胞层中 EGF 染色增加，在 36 小时时在标本中弥漫升高，然后在第 4 天角质形成细胞层的染色出现第二次高峰。EGF 的染色与其在 TM 创伤愈合中的多种作用相对应。
