O'Daniel T G, Petitjean M, Jones S C, Zogg J, Martinez S A, Nolph M B, Schultz G S
Department of Surgery, University of Louisville, Kentucky.
Ann Otol Rhinol Laryngol. 1990 Jan;99(1):80-4. doi: 10.1177/000348949009900114.
A significant percentage of tympanic membrane (TM) perforations require some form of therapy to heal. Topical application of epidermal growth factor (EGF), a potent mitogen for epidermal and mesodermal cells, promotes healing of injuries in tissues histologically similar to TM, such as cornea and skin. We evaluated TM as a target tissue for EGF action. Specific, high affinity receptors for EGF were detected in TM (Kd = 3.1 nM, 150 fmol receptor/mg protein). Autoradiography of iodine 125-EGF binding to intact TMs revealed that EGF receptors were present on cells in the stratified squamous epithelial layer and in the stromal/mucoepithelial layer. Repetitive treatment of perforated cat TMs with 20 micrograms of EGF formulated in saline induced substantial hyperplasia of epithelial and stromal layers compared to paired TMs treated with saline. A single treatment of perforated cat TMs with 50 micrograms of EGF formulated in a hydrogel or in shredded Gelfoam produced significantly (p less than .05) smaller perforations at 6 days following the operation compared to paired TMs treated with vehicles. These results demonstrate that TM is a target tissue for EGF and that topical treatment with EGF stimulates healing of TM perforations.
相当大比例的鼓膜（TM）穿孔需要某种形式的治疗才能愈合。表皮生长因子（EGF）是一种对表皮和中胚层细胞具有强大促有丝分裂作用的物质，局部应用EGF可促进组织学上与TM相似的组织（如角膜和皮肤）的损伤愈合。我们评估了TM作为EGF作用的靶组织。在TM中检测到了EGF的特异性高亲和力受体（解离常数Kd = 3.1 nM，150 fmol受体/毫克蛋白质）。用125碘标记的EGF与完整TM结合的放射自显影显示，EGF受体存在于复层鳞状上皮层和基质/黏液上皮层的细胞上。与用盐水处理的配对TM相比，用20微克溶于盐水中的EGF重复处理穿孔的猫TM可诱导上皮层和基质层的显著增生。与用载体处理的配对TM相比，用50微克溶于水凝胶或切碎的明胶海绵中的EGF单次处理穿孔的猫TM，在术后6天时穿孔明显（p < 0.05）变小。这些结果表明TM是EGF的靶组织，且局部应用EGF可刺激TM穿孔的愈合。
