Schoilew C, Velinova M, Itschev K
Zentrales Veterinärmedizinisches Forschungsinstitut, Sofia, Bulgarien.
Arch Exp Veterinarmed. 1990;44(5):655-62.
Concanavalin-A points of linkage were positively detected on cell membranes of alveolar and peritoneal macrophages by means of Con-A ferritin conjugate. Quantitative conclusions were drawn from these findings with regard to the number of mannose and glucose residues per 1 micron 2 of membrane area. With different incubation periods, 15, 25, and 45 minutes, various distribution patterns of ferritin molecules were recorded. They were diffusely distributed in cytoplasma as well as on the outer nuclear membrane. Ferritin particles were identified also on vacuolar membranes and in direct contact with lysosomes.
通过伴刀豆球蛋白A-铁蛋白偶联物在肺泡巨噬细胞和腹膜巨噬细胞的细胞膜上阳性检测到伴刀豆球蛋白A的连接点。从这些发现中得出了关于每1平方微米膜面积上甘露糖和葡萄糖残基数量的定量结论。在15分钟、25分钟和45分钟的不同孵育时间下,记录了铁蛋白分子的各种分布模式。它们在细胞质以及外核膜上呈弥散分布。在液泡膜上也鉴定出了铁蛋白颗粒,并且它们与溶酶体直接接触。