Slinkin M A, Klibanov A L, Khaw B A, Torchilin V P
U.S.S.R. Cardiology Research Center, Academy of Medical Sciences, Moscow.
Bioconjug Chem. 1990 Jul-Aug;1(4):291-5. doi: 10.1021/bc00004a011.
Modification of antibodies with chelating polymers may be helpful for radioimmunoimaging, radioimmunotherapy, and NMR tomography. Succinylated polylysine was activated with carbodiimide/N-hydroxysulfosuccinimide in dimethyl sulfoxide and isolated as a dry solid. Sulfosuccinimide-esterified polymer was used for the two-stage coupling of an amino-containing chelating agent (deferoxamine) to monoclonal R11D10 (IgG) or its Fab fragment. Conjugates were separated from free components by using gel-chromatography and anion-exchange chromatography. Antibody-coupling efficiency and the loss of its immunoreactivity upon modification have been studied for polymers with different deferoxamine content. Specific binding of 67Ga to the corresponding antigen via the conjugate has been demonstrated.
用螯合聚合物修饰抗体可能有助于放射免疫成像、放射免疫治疗和核磁共振断层扫描。琥珀酰化聚赖氨酸在二甲基亚砜中用碳二亚胺/N-羟基磺基琥珀酰亚胺活化，并作为干燥固体分离出来。磺基琥珀酰亚胺酯化的聚合物用于含氨基螯合剂（去铁胺）与单克隆R11D10（IgG）或其Fab片段的两步偶联。通过凝胶色谱和阴离子交换色谱从游离成分中分离缀合物。研究了不同去铁胺含量的聚合物的抗体偶联效率及其修饰后免疫反应性的丧失。已证明67Ga通过缀合物与相应抗原的特异性结合。
