Janoki G A, Harwig J F, Chanachai W, Wolf W
Int J Appl Radiat Isot. 1983 Jun;34(6):871-7. doi: 10.1016/0020-708x(83)90145-x.
Desferrioxamine-human serum albumin conjugate was prepared at pH 4.7 using a water-soluble carbodiimide and at pH 7.8 using glutaraldehyde. The crude conjugate was purified by dialysis and gel filtration. The efficiency of 67Ga labeling of the purified conjugate was greater than 95%, with good in vitro stability. The specific activity ranged from 5 to 50 microCi/mg. Treatment of the conjugate with urea resulted in a 20--30-fold increase in specific activity, suggesting that a number of chelon groups are unavailable for Ga binding due to non-covalent intramolecular cross-linking.
去铁胺-人血清白蛋白缀合物在pH 4.7时使用水溶性碳二亚胺制备，在pH 7.8时使用戊二醛制备。粗缀合物通过透析和凝胶过滤进行纯化。纯化后的缀合物的67Ga标记效率大于95%，具有良好的体外稳定性。比活度范围为5至50微居里/毫克。用尿素处理缀合物导致比活度增加20至30倍，这表明由于非共价分子内交联，许多螯合基团无法用于结合镓。
