Department of Chemistry, Indian Institute of Technology, Delhi, New Delhi, India.
N Biotechnol. 2011 Feb 28;28(2):136-45. doi: 10.1016/j.nbt.2010.10.007. Epub 2010 Oct 21.
Thirty-eight haloalkaliphilic bacterial strains were isolated from Sambhar Salt Lake, India and screened for their ability to secrete haloalkaliphilic proteases. Among them, a moderately halophilic, mesophilic and alkaliphilic potent strain Geomicrobium sp. EMB2 produced an extracellular protease, which was remarkably stable in organic solvents, salt, surfactants, detergents and alkaline pH. Statistically based experimental designs were applied to study the interactions and optimization of medium constituents for efficient protease production by Geomicrobium sp. EMB2. An overall 20-fold increase in protease production was achieved in the optimized medium (721 U/ml) as compared with the unoptimized medium (37 U/ml). The high production level coupled with novel properties makes it a prospective industrial enzyme. The Geomicrobium sp. EMB2 isolate is deposited in Microbial Type Culture Collection, Chandigarh, India with accession number MTCC 10310.
从印度的萨姆巴尔盐湖中分离出 38 株嗜盐嗜碱细菌菌株，并筛选它们分泌嗜盐嗜碱蛋白酶的能力。其中，一株中度嗜盐、中温、嗜碱的强菌株 Geomicrobium sp. EMB2 产生一种胞外蛋白酶，该酶在有机溶剂、盐、表面活性剂、清洁剂和碱性 pH 值中具有显著的稳定性。基于统计学的实验设计被应用于研究 Geomicrobium sp. EMB2 生产蛋白酶的培养基成分的相互作用和优化。与未优化的培养基（37 U/ml）相比，在优化的培养基（721 U/ml）中实现了蛋白酶产量的整体 20 倍增加。高产水平加上新颖的特性使其成为一种有前途的工业酶。Geomicrobium sp. EMB2 分离株保藏于印度昌迪加尔的微生物模式培养物收藏中心，登记号为 MTCC 10310。
