CIRIMAT Carnot Institute-Phosphates, Pharmacotechnics, Biomaterials, University of Toulouse, CNRS/INPT/UPS, ENSIACET, 4 allée Emile Monso, 31030 Toulouse cedex 4, France.
Colloids Surf B Biointerfaces. 2011 Feb 1;82(2):378-84. doi: 10.1016/j.colsurfb.2010.09.022.
The field of nanobiotechnology has lately attracted much attention both from therapeutic and diagnosis viewpoints. Of particular relevance is the development of colloidal formulations of biocompatible nanoparticles capable of interacting with selected cells or tissues. In this context, the purification of such nanoparticle suspensions appears as a critical step as residues of unreacted species may jeopardize biological and medical outcomes, and sample purity is thus increasingly taken into account by regulatory committees. In the present work, we have investigated from a physico-chemical point of view the purification by dialysis of recently developed hybrid colloids based on biomimetic nanocrystalline apatites intended for interacting with cells. Both Eu-doped (2mol.% relative to Ca) and Eu-free suspensions were studied. The follow-up of the dialysis process was carried out by way of FTIR, TEM, XRD, pH and conductivity measurements. Mathematical modelling of conductivity data was reported. The effects of a change in temperature (25 and 45°C), dialysis medium, and starting colloid composition were evaluated and discussed. We show that the dialysis method is a well-adapted and cheap technique to purify such mineral-organic hybrid suspensions in view of biomedical applications, and we point out some of the characterization techniques that may prove helpful for following the evolution of the purification process with time.
纳米生物技术领域最近受到了治疗和诊断观点的极大关注。特别相关的是开发能够与选定的细胞或组织相互作用的生物相容性纳米粒子的胶体制剂。在这种情况下，这种纳米粒子悬浮液的净化似乎是一个关键步骤，因为未反应的物质的残留物可能危及生物和医学结果，因此样品纯度越来越受到监管委员会的关注。在目前的工作中，我们从物理化学的角度研究了最近开发的基于仿生纳米晶磷灰石的混合胶体的透析净化，该胶体用于与细胞相互作用。研究了 Eu 掺杂（相对于 Ca 为 2mol.%）和 Eu 自由悬浮液。通过 FTIR、TEM、XRD、pH 和电导率测量来跟踪透析过程。报告了电导率数据的数学建模。评估和讨论了温度变化（25 和 45°C）、透析介质和起始胶体组成的影响。我们表明，透析方法是一种适用于生物医学应用的廉价技术，用于净化这种矿物-有机杂化悬浮液，并且我们指出了一些可能有助于随着时间的推移跟踪净化过程演变的表征技术。
