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由CD133阳性TJ905胶质母细胞瘤干细胞建立的移植瘤的病理特征。

Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

作者信息

Jin Feng, Zhang Ran, Feng Song, Yuan Chuan-Tao, Zhang Ren-Ya, Han Guang-Kui, Li Gen-Hua, Yu Xi-Zhen, Liu Yang, Kong Ling-Sheng, Zhang Shu-Ling, Zhao Lei

机构信息

Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.

Department of Pathology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029 PR China.

出版信息

Cancer Cell Int. 2015 Jun 13;15:60. doi: 10.1186/s12935-015-0208-y. eCollection 2015.

DOI:10.1186/s12935-015-0208-y
PMID:26136642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4487198/
Abstract

BACKGROUND

This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

METHODS

CD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.

RESULTS

The growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.

CONCLUSIONS

Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.

摘要

背景

本研究旨在探讨由CD133阳性的TJ905胶质母细胞瘤干细胞建立的移植瘤的病理特征。

方法

采用免疫磁珠法分离CD133阳性的TJ905胶质瘤细胞以获得胶质瘤干细胞。分别将TJ905细胞和干细胞皮下接种于小鼠，建立移植瘤模型。观察小鼠生长状况及行为。进行苏木精-伊红（HE）染色、胶质纤维酸性蛋白（GFAP）、Ki-67和少突胶质细胞转录因子2（Olig-2）的免疫组织化学检测以及CD34标记的微血管密度（MVD）检测。

结果

TJ905干细胞组小鼠的生长状况及行为表现更为明显，且模型的病理恶性特征比TJ905细胞组更强。TJ905细胞和干细胞组中的胶质纤维酸性蛋白（GFAP）表明移植瘤起源于星形胶质细胞。TJ905干细胞中Ki-67和少突胶质细胞转录因子2（Olig-2）的表达显著更高，且干细胞组中CD34的表达明显高于其他两组。

结论

由CD133阳性胶质母细胞瘤干细胞建立的移植瘤的病理特征显示出更高的恶性程度。利用TJ905干细胞在裸鼠中建立移植瘤模型对胶质瘤研究非常适用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/97988d172da1/12935_2015_208_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/c273bef073f5/12935_2015_208_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/a9e6e705d85c/12935_2015_208_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/7c6b23f174de/12935_2015_208_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/9b078d4fe58a/12935_2015_208_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/5ce163cbffc9/12935_2015_208_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/97988d172da1/12935_2015_208_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/c273bef073f5/12935_2015_208_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/a9e6e705d85c/12935_2015_208_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/7c6b23f174de/12935_2015_208_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/9b078d4fe58a/12935_2015_208_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/5ce163cbffc9/12935_2015_208_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/791d/4487198/97988d172da1/12935_2015_208_Fig6_HTML.jpg

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