Przała J, Wiesak T, Grazul A, Cieplińska E
Exp Clin Endocrinol. 1984 May;83(3):343-8. doi: 10.1055/s-0029-1210351.
Prolactin (1, 5, 10 micrograms/ml) inhibited 5 alpha-dihydrotestosterone (DHT), testosterone (T) and estradiol-17 beta (E2) secretion by luteal cells isolated from porcine corpus luteum on the 13th day of the estrous cycle. The effect of prolactin upon luteal cells on the 5th and 17th day of the cycle was different. Inhibition of DHT, T and E2 secretion in luteal cells on the 5th day of the cycle occurred only after 6 hours of incubation, at prolactin doses of 5 and 10 micrograms/ml of the medium. Luteal cells obtained from corpora lutea with considerable physiological luteolysis (17th day of the cycle) did not react to the presence of prolactin in the incubation medium. The results showed that the effect of prolactin upon luteal cells depended on the intensity of steroidogenesis. Luteal cells on the 5th day of the estrous cycle produced progesterone in the amounts 34 +/- 9.1 ng/ml, on the 13th day: 62 +/- 11.5, and on the 17th day: 7.9 +/- 1.9. The effect of prolactin was most pronounced in case of cells on the 13th day of the cycle. These cells were characterized by the highest production of progesterone. The in vitro observations suggest the role of prolactin in inhibiting synthesis or release of DHT, T and E2 from luteal cells.
催乳素（1、5、10微克/毫升）抑制了从发情周期第13天的猪黄体分离出的黄体细胞分泌5α-二氢睾酮（DHT）、睾酮（T）和雌二醇-17β（E2）。催乳素对周期第5天和第17天黄体细胞的作用有所不同。仅在以5和10微克/毫升的催乳素剂量培养6小时后，周期第5天的黄体细胞中DHT、T和E2的分泌才受到抑制。从具有明显生理性黄体溶解的黄体（周期第17天）获得的黄体细胞对培养液中催乳素的存在没有反应。结果表明，催乳素对黄体细胞的作用取决于类固醇生成的强度。发情周期第5天的黄体细胞产生的孕酮量为34±9.1纳克/毫升，第13天为62±11.5，第17天为7.9±1.9。催乳素的作用在周期第13天的细胞中最为明显。这些细胞的特征是孕酮产量最高。体外观察结果提示催乳素在抑制黄体细胞合成或释放DHT、T和E2方面发挥作用。
