Institut universitaire de cardiologie et de pneumologie de Québec (Hôpital Laval), 2725 chemin Sainte-Foy, Québec, Canada G1V 4G5.
Int J Hyg Environ Health. 2010 Nov;213(6):444-9. doi: 10.1016/j.ijheh.2010.09.008. Epub 2010 Oct 23.
Swine confinement buildings in eastern Canada are enclosed and equipped with modern production systems to manage waste. Bioaerosols of these swine confinement buildings could be contaminated by human pathogens and antimicrobial resistant bacteria which could colonize exposed workers. We therefore wanted to analyze bioaerosols of swine confinement buildings and nasal flora of Canadian hog producers to evaluate possible colonization with human pathogens and tetracycline-resistant bacteria. Culturable and non-culturable human pathogens and tet genes were investigated in the bioaerosols of 18 barns. The nasal passages of 35 hog producers were sampled and total DNA was extracted from the calcium-alginate swabs to detect, by PCR, Campylobacter, C. perfringens, Enterococcus, E. coli, Y. enterocolitica, tetA/tetC, tetG and ribosomal protection protein genes. Airborne culturable C. perfringens, Enterococcus, E. coli, and Y. enterocolitica were present in the bioaerosols of 16, 17, 11 and 6 of the 18 facilities. Aerosolized total (culturable/non culturable) Campylobacter, C. perfringens, Enterococcus, E. coli and Y. enterocolitica were detected in 10, 6, 15, 18 and 2 barns, respectively. Tet genes were found in isolates of culturable human pathogens. TetA/tetC, tetG and ribosomal protection protein genes were detected in the bioaerosols of all 18 studied buildings. Campylobacter, C. perfringens, Enterococcus, E. coli, and Y. enterocolitica were found respectively in 4, 9, 17, 14 and one nasal flora of workers. One and 10 workers were positive for tetA/tetC and tetG genes, respectively. In swine confinement buildings, hog producers are exposed to aerosolized human pathogens and tetracycline-resistant bacteria that can contaminate the nasal flora.
加拿大东部的养猪场被封闭，并配备了现代生产系统来处理废物。这些养猪场的生物气溶胶可能被人类病原体和抗微生物耐药细菌污染，这些细菌可能会在暴露的工人中定植。因此，我们希望分析养猪场的生物气溶胶和加拿大养猪户的鼻腔菌群，以评估可能与人类病原体和四环素耐药菌的定植情况。我们调查了 18 个畜舍的生物气溶胶中的可培养和不可培养的人类病原体和 tet 基因。从 35 名养猪户的鼻腔中取样，并从钙-海藻酸钠拭子中提取总 DNA，通过 PCR 检测弯曲杆菌、产气荚膜梭菌、肠球菌、大肠杆菌、小肠结肠炎耶尔森菌、tetA/tetC、tetG 和核糖体保护蛋白基因。在 18 个设施中的 16、17、11 和 6 个设施的生物气溶胶中存在可培养的产气荚膜梭菌、肠球菌、大肠杆菌和小肠结肠炎耶尔森菌。在 10、6、15、18 和 2 个畜舍中分别检测到气溶胶化的总（可培养/不可培养）弯曲杆菌、产气荚膜梭菌、肠球菌、大肠杆菌和小肠结肠炎耶尔森菌。在可培养的人类病原体分离株中发现了 tet 基因。tetA/tetC、tetG 和核糖体保护蛋白基因在所有 18 个研究建筑物的生物气溶胶中均有检测到。在分别在 4、9、17、14 和 1 名工人的鼻腔菌群中发现了弯曲杆菌、产气荚膜梭菌、肠球菌、大肠杆菌和小肠结肠炎耶尔森菌。有 1 人和 10 人分别对 tetA/tetC 和 tetG 基因呈阳性。在养猪场中，养猪户暴露于气溶胶化的人类病原体和四环素耐药细菌中，这些细菌会污染鼻腔菌群。
