Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK.
J Cell Sci. 2010 Nov 1;123(Pt 21):3629-38. doi: 10.1242/jcs.064485.
Adhesion is fundamental to the survival and function of many different cell types, and regulates basic events such as mitosis, cell survival and migration, in both embryonic and adult organisms. Cell-matrix adhesion also regulates the dynamic interplay between cells and surrounding tissues during processes such as immune cell recruitment, wound healing and cancer cell metastasis. The study of cell adhesion has gained momentum in recent years, in large part because of the emergence of imaging techniques that have facilitated detailed analysis of the molecular composition and dynamics of the structures involved. In this Commentary, we discuss the recent application of different imaging techniques to study cell-matrix adhesions, emphasising common strategies used for the analysis of adhesion dynamics both in cells in culture and in whole organisms.
黏附是许多不同类型细胞存活和功能的基础，并且调节有丝分裂、细胞存活和迁移等基本事件，在胚胎和成年生物中都是如此。细胞-基质黏附还调节免疫细胞募集、伤口愈合和癌细胞转移等过程中细胞与周围组织之间的动态相互作用。近年来，细胞黏附的研究进展迅速，在很大程度上是因为成像技术的出现，这些技术促进了对涉及的结构的分子组成和动态的详细分析。在本评论中，我们讨论了不同成像技术在研究细胞-基质黏附中的最新应用，强调了用于分析培养细胞和整个生物体中黏附动态的常用策略。
