Zhou Ping, Ding Lurong, Yan Yajuan, Wang Yafeng, Su Bin
Key Laboratory of Excited-State Materials of Zhejiang Province, Institute of Analytical Chemistry, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.
Chem Commun (Camb). 2023 Feb 23;59(17):2341-2351. doi: 10.1039/d2cc06499e.
Cell-matrix adhesions play an essential role in mediating and regulating many biological processes. The adhesion receptors, typically transmembrane integrins, provide dynamic correlations between intracellular environments and extracellular matrixes (ECMs) by bi-directional signaling. In-depth investigations of cell-matrix adhesion and integrin-mediated cell adhesive force are of great significance in biology and medicine. The emergence of advanced imaging techniques and principles has facilitated the understanding of the molecular composition and structure dynamics of cell-matrix adhesions, especially the label-free imaging methods that can be used to study living cell dynamics without immunofluorescence staining. This highlight article aims to give an overview of recent developments in imaging cell-matrix adhesions in a label-free manner. Electrochemiluminescence microscopy (ECLM) and surface plasmon resonance microscopy (SPRM) are briefly introduced and their applications in imaging analysis of cell-matrix adhesions are summarized. Then we highlight the advances in mapping cell-matrix adhesion force based on molecular tension probes and fluorescence microscopy (collectively termed as MTFM). The biomaterials including polyethylene glycol (PEG), peptides and DNA for constructing tension probes in MTFM are summarized. Finally, the outlook and perspectives on the further developments of cell-matrix adhesion imaging are presented.
细胞与基质的黏附在介导和调节许多生物学过程中起着至关重要的作用。黏附受体，通常是跨膜整合素，通过双向信号传导在细胞内环境和细胞外基质（ECM）之间提供动态关联。对细胞与基质黏附以及整合素介导的细胞黏附力进行深入研究在生物学和医学中具有重要意义。先进成像技术和原理的出现促进了对细胞与基质黏附的分子组成和结构动力学的理解，尤其是那些无需免疫荧光染色即可用于研究活细胞动力学的无标记成像方法。这篇重点文章旨在概述以无标记方式成像细胞与基质黏附的最新进展。简要介绍了电化学发光显微镜（ECLM）和表面等离子体共振显微镜（SPRM），并总结了它们在细胞与基质黏附成像分析中的应用。然后我们重点介绍基于分子张力探针和荧光显微镜（统称为MTFM）绘制细胞与基质黏附力图谱的进展。总结了用于在MTFM中构建张力探针的包括聚乙二醇（PEG）、肽和DNA在内的生物材料。最后，对细胞与基质黏附成像的进一步发展进行了展望和展望。
