Capital Medical University, Beijing 100069, China.
Endocrine. 2010 Dec;38(3):360-8. doi: 10.1007/s12020-010-9389-2. Epub 2010 Oct 23.
Prolactinomas are the most common secretory pituitary tumors; however, their pathogenesis is unclear. In order to explore the pathogenesis of prolactinomas, we used fiber-optic BeadArray to examine gene expression profiles in five prolactinomas compared with three normal pituitaries. Three down-regulated genes and one up-regulated gene were chosen for validation by quantitative real-time reverse-transcription polymerase chain reaction. We then performed pathway analysis on the identified differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Array analysis showed significant increases in the expression of 27 genes and 3 expressed sequence tags (ESTs), and decreases in 182 genes and 9 ESTs, including HIG1 domain family, member 1B, S100 calcium binding protein A9, angiopoietin 2, interleukin 8, hydroxyprostaglandin dehydrogenase 15-(NAD), suppression of tumorigenicity18, and WNT inhibitory factor 1. Pathway analysis showed that the P53 and GnRH signaling pathways may play an important role in tumorigenesis of prolactinomas. Our data suggest fiber-optic BeadArray combined with pathway analysis of differential gene expression profile appears to be a valid approach for investigating the pathogenesis of tumors.
催乳素瘤是最常见的分泌性垂体肿瘤；然而，其发病机制尚不清楚。为了探讨催乳素瘤的发病机制，我们使用光纤珠阵列比较了 5 例催乳素瘤与 3 例正常垂体的基因表达谱。选择了三个下调基因和一个上调基因，通过实时定量逆转录聚合酶链反应进行验证。然后，我们使用京都基因与基因组百科全书对鉴定出的差异表达基因进行了途径分析。阵列分析显示，27 个基因和 3 个表达序列标签（EST）的表达显著增加，182 个基因和 9 个 EST 的表达减少，包括 HIG1 结构域家族成员 1B、S100 钙结合蛋白 A9、血管生成素 2、白细胞介素 8、羟前列腺素脱氢酶 15-(NAD)、肿瘤抑制基因 18 和 WNT 抑制因子 1。途径分析表明，P53 和 GnRH 信号通路可能在催乳素瘤的发生中起重要作用。我们的数据表明，光纤珠阵列结合差异基因表达谱的途径分析似乎是研究肿瘤发病机制的有效方法。
