BioEnergy Science Center, Georgia Institute of Technology, 500 10th St., Atlanta, GA 30332, USA.
Rapid Commun Mass Spectrom. 2010 Nov 30;24(22):3230-6. doi: 10.1002/rcm.4757.
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was applied to the analysis of the spatial distribution of cellulose on a cross-section of juvenile poplar (Populus deltoids) stems. Microcrystalline cellulose (MCC) was used to optimize matrix (2,5-dihydroxybenzoic acid) application and instrument parameters for the detection of low hexose oligomers, which originated from cellulose in the solid phase. A section of poplar cellulose isolated from juvenile poplar stem which consisted primarily of glucose (∼95%) and minor components such as xylose and lignin was used for the MALDI-IMS studies. The mass spectrum of poplar cellulose consisted of a series of evenly spaced signals having a difference of 162 m/z units, which was similar to that of MCC in linear and reflectron positive ion modes. MS images of cellulose compounds with sodium ion adducts were generated and illustrated the distribution of cellulose on the surface of the poplar stem.
基质辅助激光解吸/电离成像质谱 (MALDI-IMS) 被应用于分析幼龄白杨 (Populus deltoids) 茎横截面上纤维素的空间分布。微晶纤维素 (MCC) 被用于优化基质 (2,5-二羟基苯甲酸) 的应用和仪器参数，以检测来自固相纤维素的低己糖低聚物。从小麦纤维素中分离出来的一小段白杨纤维素，主要由葡萄糖（约 95%）和少量成分如木糖和木质素组成，用于 MALDI-IMS 研究。白杨纤维素的质谱由一系列均匀间隔的信号组成，其质荷比差异为 162 m/z 单位，这与线性和反射正离子模式下的 MCC 相似。生成了带有钠离子加合物的纤维素化合物的 MS 图像，并说明了纤维素在白杨茎表面的分布。
