Department of Obstetrics and Gynecology, Tokyo Medical University Division of Reproductive Medicine, Sugiyama Clinic, Tokyo, Japan.
Hum Cell. 2010 Aug;23(3):83-8. doi: 10.1111/j.1749-0774.2010.00088.x. Epub 2010 Oct 4.
The aim of this study was to determine whether the bone-morphogenetic proreins-2 (BMP-2) or -7 (BMP-7) levels in follicular fluid (FF) are associated with success in oocyte fertilization during assisted reproductive technology (ART) treatment. Twenty-four cycles in 24 patients who underwent oocyte retrieval in Sugiyama clinic were included in this retrospective study. The patients were divided into two groups according to the success or failure of fertilization. FF samples were obtained from a single follicle in each patient, and the levels of BMP-2, BMP-7, Anti-Müllerian hormone (AMH), estradiol and progesterone from FF were measured, and evaluated in relation to the ART outcomes. The BMP-2 levels correlated positively with the AMH levels in FF (r(2) = 0.4928), but there was no statistically significant difference between BMP-7 and AMH levels. The BMP-2 and BMP-7 levels had no relation with either progesterone or estradiol levels, but BMP-2 levels in the fertilized group were significantly higher than those in the unfertilized group (P < 0.05). The BMP-2 levels in FF positively correlated with the AMH levels in FF, and those in the fertilized group were significantly higher than in the unfertilized group. Therefore, the BMP-2 levels in FF could be a predictive marker for fertilization.
本研究旨在确定卵泡液（FF）中的骨形态发生蛋白-2（BMP-2）或 -7（BMP-7）水平是否与辅助生殖技术（ART）治疗中卵母细胞受精成功相关。本回顾性研究纳入了在杉山诊所接受卵母细胞采集的 24 名患者的 24 个周期。根据受精成功与否，将患者分为两组。从每位患者的单个卵泡中获取 FF 样本，并测量 FF 中的 BMP-2、BMP-7、抗苗勒氏管激素（AMH）、雌二醇和孕酮水平，并评估其与 ART 结果的关系。FF 中的 BMP-2 水平与 AMH 水平呈正相关（r(2) = 0.4928），但 BMP-7 和 AMH 水平之间无统计学差异。BMP-2 和 BMP-7 水平与孕酮或雌二醇水平均无关系，但受精组的 BMP-2 水平明显高于未受精组（P < 0.05）。FF 中的 BMP-2 水平与 FF 中的 AMH 水平呈正相关，且受精组明显高于未受精组。因此，FF 中的 BMP-2 水平可能是受精的预测标志物。
