Tramišak Milaković Tamara, Panić Horvat Linda, Čavlović Kristina, Smiljan Severinski Neda, Vlašić Hrvoje, Vlastelić Ivan, Ljiljak Dejan, Radojčić Badovinac Anđelka
Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka, University of Rijeka, Cambierieva 17/5, 51 000, Rijeka, Croatia,
Arch Gynecol Obstet. 2015 Mar;291(3):681-7. doi: 10.1007/s00404-014-3460-9. Epub 2014 Sep 10.
The present study aimed to correlate anti-Müllerian hormone (AMH) levels in follicular fluid (FF) with oocyte maturity stages, morphological quality of metaphase II (MII) oocyte and fertilization capacity of MII oocytes.
A total of 92 infertile women undergoing controlled ovarian stimulation and intracytoplasmic sperm injection were analyzed. Patients were divided into two groups according to age: <35 years (n = 43) and ≥35 years (n = 49). An FF sample was obtained from a single dominant follicle in each patient for a total of 92 follicular fluid samples analyzed. AMH levels in serum and follicular fluid were measured by enzyme-linked immunosorbent assay. Mature MII oocytes, zygotes, and embryos were assessed for morphological quality.
Serum AMH levels were significantly higher in patients aged <35 years. No correlation was observed between FF AMH level and oocyte maturation stages or morphological quality of MII oocyte. Significantly lower FF AMH levels were observed in fertilized MII oocytes than in non-fertilized MII oocytes in patients aged <35 years (2.56 ± 2.0 ng/ml vs. 4.81 ± 4.14 ng/ml; p = 0.032).
The present study revealed no correlation between FF AMH and oocyte maturity stage or morphological quality of MII oocyte. However, FF AMH might be a predictive marker for fertilization capacity of MII oocytes.
本研究旨在探讨卵泡液（FF）中抗苗勒管激素（AMH）水平与卵母细胞成熟阶段、中期II（MII）卵母细胞形态质量及MII卵母细胞受精能力之间的相关性。
对92例行控制性卵巢刺激和卵胞浆内单精子注射的不孕妇女进行分析。根据年龄将患者分为两组：<35岁（n = 43）和≥35岁（n = 49）。从每位患者的单个优势卵泡中获取FF样本，共分析92份卵泡液样本。采用酶联免疫吸附测定法测量血清和卵泡液中的AMH水平。对成熟的MII卵母细胞、受精卵和胚胎进行形态质量评估。
<35岁患者的血清AMH水平显著更高。未观察到卵泡液AMH水平与卵母细胞成熟阶段或MII卵母细胞形态质量之间的相关性。在<35岁的患者中，受精的MII卵母细胞的卵泡液AMH水平显著低于未受精的MII卵母细胞（2.56±2.0 ng/ml对4.81±4.14 ng/ml；p = 0.032）。
本研究表明卵泡液AMH与卵母细胞成熟阶段或MII卵母细胞形态质量之间无相关性。然而，卵泡液AMH可能是MII卵母细胞受精能力的预测指标。
