Immunology Research Centre, Department of Medicine, St. Vincent's Hospital, The University of Melbourne, Melbourne, Victoria, Australia.
Am J Transplant. 2010 Nov;10(11):2410-20. doi: 10.1111/j.1600-6143.2010.03291.x.
We have shown that CD39 and CD73 are coexpressed on the surface of murine CD4+ Foxp3+ regulatory T cells (Treg) and generate extracellular adenosine, contributing to Treg immunosuppressive activity. We now describe that CD39, independently of CD73, is expressed by a subset of blood-derived human CD4+ CD25+ CD127lo Treg, defined by robust expression of Foxp3. A further distinct population of CD4+ CD39+ T lymphocytes can be identified, which do not express CD25 and FoxP3 and exhibit the memory effector cellular phenotype. Differential expression of CD25 and CD39 on circulating CD4+ T cells distinguishes between Treg and pathogenic cellular populations that secrete proinflammatory cytokines such as IFNγ and IL-17. These latter cell populations are increased, with a concomitant decrease in the CD4+ CD25+ CD39+ Tregs, in the peripheral blood of patients with renal allograft rejection. We conclude that the ectonucleotidase CD39 is a useful and dynamic lymphocytes surface marker that can be used to identify different peripheral blood T cell-populations to allow tracking of these in health and disease, as in renal allograft rejection.
我们已经表明，CD39 和 CD73 在鼠源 CD4+ Foxp3+ 调节性 T 细胞（Treg）的表面上共表达，并产生细胞外腺苷，有助于 Treg 的免疫抑制活性。我们现在描述的是，CD39 独立于 CD73，由血液来源的人 CD4+ CD25+ CD127lo Treg 的一个亚群表达，该亚群以 Foxp3 的强表达为特征。可以鉴定出另一群独特的 CD4+ CD39+ T 淋巴细胞，它们不表达 CD25 和 FoxP3，并表现出记忆效应细胞表型。循环 CD4+ T 细胞上 CD25 和 CD39 的差异表达可区分分泌促炎细胞因子（如 IFNγ 和 IL-17）的 Treg 和致病性细胞群体。在肾移植排斥患者的外周血中，这些后一种细胞群体增加，同时 CD4+ CD25+ CD39+ Tregs 减少。我们得出结论，胞外核苷酸酶 CD39 是一个有用的、动态的淋巴细胞表面标志物，可用于识别不同的外周血 T 细胞群体，以在健康和疾病中（如肾移植排斥）追踪这些群体。
