Department of Physiology and Zoology, Faculty of Pharmacy, Seville University, C/ Profesor García González s/n° 2, Seville, Spain.
Alcohol Alcohol. 2010 Nov-Dec;45(6):489-94. doi: 10.1093/alcalc/agq067. Epub 2010 Oct 25.
Levels of antioxidants such as folic acid and selenium decrease in dams exposed to ethanol during gestation and lactation, affecting their antioxidant status, their reproductive function and consequently the health of their progeny. We will study whether a Se (0.5 p.p.m.) plus folic acid (8 p.p.m.) supplemented diet administered to ethanol-exposed dams and male rats prevents the effects provoked by ethanol in Se bioavailability and in their glutathione peroxidase (GPx) activity, thus improving the health of their offspring.
Se levels in tissue were measured by graphite-furnace atomic absorption spectrometry and serum GPx activity by spectrophotometry.
Results show that ethanol decreases Se retention in dams, affecting their tissues' Se deposits, decreasing serum GPx activity, gestational parameters and the weight of their progeny.
Se plus folic acid balance Se bioavailability, something that is especially important during gestation and lactation, and as a direct result, the health of their progeny is improved.
在妊娠和哺乳期暴露于乙醇的母鼠体内，抗氧化剂（如叶酸和硒）的水平会降低，这会影响其抗氧化状态、生殖功能，进而影响其后代的健康。我们将研究在暴露于乙醇的母鼠和雄性大鼠中添加（0.5ppm）硒和（8ppm）叶酸的饮食是否可以防止乙醇对硒生物利用度和谷胱甘肽过氧化物酶（GPx）活性的影响，从而改善其后代的健康。
采用石墨炉原子吸收光谱法测定组织中的硒水平，采用分光光度法测定血清 GPx 活性。
结果表明，乙醇降低了母鼠体内的硒保留，影响了其组织中的硒沉积，降低了血清 GPx 活性、妊娠参数和后代的体重。
硒和叶酸的补充平衡了硒的生物利用度，这在妊娠和哺乳期尤为重要，因此，改善了其后代的健康。
