[Production and characterization of a high affinity monoclonal antibody with digoxin and digitoxin specificity].
作者信息
Simon H U, Hubl W, Müller W D, Wolf I, Schlenvoigt G, Jäger L
机构信息
Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
出版信息
Allerg Immunol (Leipz). 1990;36(4):343-50.
PMID:2097902
Abstract
A monoclonal antibody with a high affinity for digoxin (KA = 5.5 x 10(10) M-1) and digitoxin KA = 5.0 x 10(10) M-1) was produced by somatic cell fusion. This antibody, designated 2A3, displayed little cross reactivity with other glucosides and no cross reactivity with endogenous steroids. It was shown that 2A3 is a suitable tool in an enzyme immunoassay for digoxin and digitoxin.
摘要
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引用本文的文献
1
Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes.在培养的大鼠心肌细胞测试系统中，地高辛单克隆抗体的功能性拮抗作用。
Mol Cell Biochem. 1996 Jul-Aug;160-161:117-20. doi: 10.1007/BF00240040.
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