Simon H U, Hubl W, Müller W D, Wolf I, Schlenvoigt G, Jäger L
Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
Allerg Immunol (Leipz). 1990;36(4):343-50.
A monoclonal antibody with a high affinity for digoxin (KA = 5.5 x 10(10) M-1) and digitoxin KA = 5.0 x 10(10) M-1) was produced by somatic cell fusion. This antibody, designated 2A3, displayed little cross reactivity with other glucosides and no cross reactivity with endogenous steroids. It was shown that 2A3 is a suitable tool in an enzyme immunoassay for digoxin and digitoxin.
