地高辛单克隆抗体对豚鼠心房的功能性拮抗作用。
Functional antagonism by monoclonal antibody to digoxin in the guinea-pig atria.
作者信息
Wong P C, Reilly T M, Karol R A, Timmermans P B
出版信息
Eur J Pharmacol. 1987 Apr 29;136(3):437-40. doi: 10.1016/0014-2999(87)90321-9.
A monoclonal antibody with a high affinity for digoxin (Ka = 1.14 X 10(9) M) was produced by somatic cell fusion. This antibody, designated 35/6.1, displayed little or no cross reactivity with other steroids. In the guinea-pig atria, 35/6.1 antagonized functionally the positive inotropic effect of digoxin but did not alter that of isoproterenol. Our results suggest that this monoclonal antibody may represent a useful probe for studying the physiological role of the putative endogenous digoxin-like factor in various pathophysiological conditions.
通过体细胞融合制备了一种对地高辛具有高亲和力（Ka = 1.14×10⁹ M）的单克隆抗体。这种抗体命名为35/6.1，与其他甾体几乎没有交叉反应。在豚鼠心房中，35/6.1在功能上拮抗地高辛的正性肌力作用，但不改变异丙肾上腺素的正性肌力作用。我们的结果表明，这种单克隆抗体可能是研究假定的内源性地高辛样因子在各种病理生理状况下生理作用的有用探针。
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