Li Rong, Luo Yuxiao, Dong Qin, Yin Yuqing, Ma Yiwei, Pan Jiayu, Pan Yaping, Zhang Dongmei
Department of Periodontology, Shenzhen Stomatological Hospital, Southern Medical University, Shenzhen, Guangdong 518000, P.R. China.
Department of Periodontology, School of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Disease, Shenyang, Liaoning 110002, P.R. China.
Exp Ther Med. 2023 Sep 4;26(4):489. doi: 10.3892/etm.2023.12188. eCollection 2023 Oct.
Whether () infection is associated with periodontitis has been contested for decades. The relationship between genotypes and periodontitis has not been clarified either. The present study provides a novel perspective to better understand the role of in the pathogenesis of periodontitis. A total of 53 volunteers were recruited and divided into 3 groups in this cross-sectional study, namely the periodontally healthy group (15 participants), the stage I/II periodontitis group (20 participants) and the stage III/IV periodontitis group (18 participants). DNA from the subgingival plaque of all participants was extracted and PCR was performed using specific primers for the urease C gene and cytotoxin-associated gene A ()/vacuolating cytotoxin gene A () to detect the presence and genotype of . A χ test and one-way ANOVA were performed on the data. There was no significant difference in sex, age or body mass index between the groups. The detection rate of was 39.62% in the total population and increased with the deepening of probing depth and clinical attachment loss. There were significant differences in the detection rate of among the three groups, with 13.33, 40.00 and 61.11% in the periodontally healthy, stage I/II periodontitis and stage III/IV periodontitis groups, respectively (χ=8.760, P<0.001). The /s2m2 genotype was most commonly detected in the periodontally healthy group (100%). In the periodontitis group, /s1m2 was the most commonly detected genotype in the stage I/II periodontitis group (37.5%) and /s1m1 in the stage III/IV periodontitis group (36.3%). The results of the present study suggest that the detection rates and genotypes of in the subgingival plaque are associated with the status of periodontitis. /s1m1 and /s1m2 may be considered virulence markers of periodontitis. However, given the small sample size and lack of correlation analysis of the study, further larger scale and high-quality clinical trials are required to confirm these findings.
几十年来，关于（）感染是否与牙周炎相关一直存在争议。（）基因型与牙周炎之间的关系也尚未阐明。本研究提供了一个新的视角，以更好地理解（）在牙周炎发病机制中的作用。在这项横断面研究中，共招募了53名志愿者并将其分为3组，即牙周健康组（15名参与者）、I/II期牙周炎组（20名参与者）和III/IV期牙周炎组（18名参与者）。提取所有参与者龈下菌斑的DNA，并使用脲酶C基因和细胞毒素相关基因A（）/空泡毒素基因A（）的特异性引物进行聚合酶链反应（PCR），以检测（）的存在和基因型。对数据进行χ检验和单因素方差分析。各组之间在性别、年龄或体重指数方面无显著差异。总体人群中（）的检出率为39.62%，且随着探诊深度加深和临床附着丧失而增加。三组之间（）的检出率存在显著差异，牙周健康组、I/II期牙周炎组和III/IV期牙周炎组分别为13.33%、40.00%和61.11%（χ = 8.760，P < 0.001）。/s2m2基因型在牙周健康组中最常被检测到（100%）。在牙周炎组中，/s1m2是I/II期牙周炎组中最常被检测到的基因型（37.5%），而在III/IV期牙周炎组中/s1m1是最常被检测到的基因型（36.3%）。本研究结果表明，龈下菌斑中（）的检出率和基因型与牙周炎状况相关。/s1m1和/s1m2可能被视为牙周炎的毒力标志物。然而，鉴于本研究样本量小且缺乏相关性分析，需要进一步开展更大规模和高质量的临床试验来证实这些发现。
