Xiao Su-Kun, Zhao Chun-Jiang, Liu Chun-Lin, Wang Hui
Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2010 Aug;33(8):601-7.
To investigate the serogroups/types distribution and antimicrobial susceptibility of clinical Streptococcus pneumoniae isolates of different serogroups/types in both children and adults in China, and to explore the significance of vaccines in preventing pneumococcal infections and control of epidemic Streptococcus pneumoniae.
A total of 580 consecutive and non-repetitive Streptococcus pneumoniae isolates were collected from 13 hospitals between 2005 and 2008. Agar dilution method was used to determine the minimal inhibitory concentrations (MIC) of 11 antibacterial agents. Serotyping was performed by latex agglutination test and the Quellung reaction test.
The most prevalent serogroups/types in 362 isolates from adults were 19F (55, 15.2%), 19A (46, 12.7%), 3 (44, 12.2%), 23F (24, 6.6%), 15 (23, 6.4%), and 17 (11, 3.0%), while in the 218 isolates from children, 19F (71, 32.6%), 19A (31, 14.2%), 23F (13, 6.0%), 15 (12, 5.5%), 14 (11, 5.0%), and 6B (10, 4.6%) were the most prevalent. Resistance to β-lactams was related to the serotypes. 19F and 19A were more resistant toβ-lactams than the other serotypes. The prevalence of Penicillin Intermediate Streptococcus pneumoniae increased from 7.4% in 2005 to 24.9% in 2008. The coverage of pneumococcal conjugate vaccine (PCV)-7, PCV-10 and PCV-13 among all age groups was 35.5% (206/580), 38.7% (224/580) and 61.8% (358/580), respectively. The coverage of PCV-7, PCV-10 and PCV-13 among children under 5 years was 55.7% (78/140), 58.6% (82/140), and 77.9% (109/140) respectively.
Penicillin intermediate isolates were on the rise with years. PCV-7, PCV-10 and PCV-13 vaccines showed a higher coverage in children than in adults.
调查中国不同血清型的临床肺炎链球菌分离株在儿童和成人中的血清群/型分布及抗菌药物敏感性，探讨疫苗在预防肺炎球菌感染和控制肺炎链球菌流行中的意义。
2005年至2008年期间，从13家医院收集了580株连续且无重复的肺炎链球菌分离株。采用琼脂稀释法测定11种抗菌药物的最低抑菌浓度（MIC）。通过乳胶凝集试验和荚膜肿胀反应试验进行血清分型。
362株成人分离株中最常见的血清群/型为19F（55株，15.2%）、19A（46株，12.7%）、3（44株，12.2%）、23F（24株，6.6%）、15（23株，6.4%）和17（11株，3.0%），而在218株儿童分离株中，最常见的是19F（71株，32.6%）、19A（31株，14.2%）、23F（13株，6.0%）、15（12株，5.5%）、14（11株，5.0%）和6B（10株，4.6%）。对β-内酰胺类药物的耐药性与血清型有关。19F和19A比其他血清型对β-内酰胺类药物更耐药。青霉素中介型肺炎链球菌的患病率从2005年的7.4%上升至2008年的24.9%。肺炎球菌结合疫苗（PCV）-7、PCV-10和PCV-13在所有年龄组中的覆盖率分别为35.5%（206/580）、38.7%（224/580）和61.8%（358/580）。5岁以下儿童中PCV-7、PCV-10和PCV-13的覆盖率分别为55.7%（78/140）、58.6%（82/140）和77.9%（109/140）。
青霉素中介型分离株逐年增多。PCV-7、PCV-10和PCV-13疫苗在儿童中的覆盖率高于成人。
