Yang Ya-bing, Li Pei, Liu Mei-lin
Department of Geriatrics, Peking University First Hospital, Beijing 100034, China.
Zhonghua Yi Xue Za Zhi. 2010 Jul 27;90(28):2004-7.
To investigate the effect of n-3 polyunsaturated fatty acids (n-3PUFAs) on the hemorheology and coagulation function of high-fat induced atherosclerotic rats and understand the underlying mechanism.
Twenty-four Wistar rats were assigned randomly into 3 groups: normal control, model and n-3PUFAs treatment (n = 8 in each). The rats in model and treatment groups were injected with a single dose of vitamin D(3) (600,000 U/kg) and fed with a high-fat diet. Basic chow was provided for normal control group. After a 6-week high-fat diet, the rats in treatment group were treated with n-3PUFAs at 250 mg×kg(-1)×d(-1) by gastric tube. The serum lipid, aortal morphological changes, hemorheology, coagulation, nitric oxide (NO), total antioxidant capacity (T-AOC) and malonaldehyde (MDA) were detected after a 6-week n-3PUFAs diet.
Compared with control group, model group rat total cholesterol (TC), low density cholesterol (LDL-C), plasma viscosity, whole blood viscosity, fibrinogen (FIB) and MDA concentrations were higher (all P < 0.05), but activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), erythrocyte deformation index(DI), plasma NO and T-AOC were lower (all P < 0.05). Compared with model group, n-3PUFAs could reduce blood lipid levels, inhibit atherosclerotic plaque formation, decrease plasma viscosity [(1.58 ± 0.23) mPa·s vs (1.81 ± 0.16) mPa·s], whole blood viscosity [(4.76 ± 0.42) mPa·s vs (5.47 ± 0.41) mPa·s, (4.24 ± 0.32) mPa·s vs (4.91 ± 0.39) mPa·s, (4.04 ± 0.29) mPa·s vs (4.58 ± 0.33) mPa·s] and FIB [(2.45 ± 0.12)g/L vs (2.65 ± 0.13) g/L], lower MDA content [(10.1 ± 0.7) µmol/ml vs (11.2 ± 0.6) µmol/ml], prolong APTT, PT and TT [(29.04 ± 0.49)s vs (26.46 ± 0.25) s, (13.86 ± 0.55) s vs (10.71 ± 0.34) s, (23.05 ± 0.24) s vs (20.90 ± 0.68) s], increase erythrocyte DI (0.35 ± 0.01 vs 0.31 ± 0.02), plasma NO [(3.9 ± 0.7) nmol/ml vs (2.8 ± 0.7) nmol/ml] and T-AOC levels [(8.0 ± 0.6) U/ml vs (6.7 ± 0.6) U/ml]of atherosclerotic rats (all P < 0.05).
n-3PUFAs may improve hemorheology and coagulation of atherosclerotic rats, reduce oxidative stress, improve endothelial function and inhibit atherosclerotic plaque formation.
探讨n-3多不饱和脂肪酸（n-3PUFAs）对高脂诱导的动脉粥样硬化大鼠血液流变学及凝血功能的影响，并了解其潜在机制。
将24只Wistar大鼠随机分为3组：正常对照组、模型组和n-3PUFAs治疗组（每组8只）。模型组和治疗组大鼠单次注射维生素D(3)（60万U/kg）并给予高脂饮食。正常对照组给予基础饲料。高脂饮食6周后，治疗组大鼠通过灌胃给予n-3PUFAs，剂量为250 mg×kg(-1)×d(-1)。n-3PUFAs饮食6周后，检测血清脂质、主动脉形态学变化、血液流变学、凝血指标、一氧化氮（NO）、总抗氧化能力（T-AOC）和丙二醛（MDA）。
与对照组相比，模型组大鼠总胆固醇（TC）、低密度胆固醇（LDL-C）、血浆黏度、全血黏度、纤维蛋白原（FIB）和MDA浓度均升高（均P < 0.05），但活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、凝血酶时间（TT）、红细胞变形指数（DI）、血浆NO和T-AOC均降低（均P < 0.05）。与模型组相比，n-3PUFAs可降低血脂水平，抑制动脉粥样硬化斑块形成，降低血浆黏度[（1.58±0.23）mPa·s对（1.81±0.16）mPa·s]、全血黏度[（4.76±0.42）mPa·s对（5.47±0.41）mPa·s，（4.24±0.32）mPa·s对（4.91±0.39）mPa·s，（4.04±0.29）mPa·s对（4.58±0.33）mPa·s]和FIB[（2.45±0.12）g/L对（2.65±0.13）g/L]，降低MDA含量[（10.1±0.7）µmol/ml对（11.2±0.6）µmol/ml]，延长APTT、PT和TT[（29.04±0.49）s对（26.46±0.25）s，（13.86±0.55）s对（10.71±0.34）s，（23.05±0.24）s对（20.90±0.68）s]，增加动脉粥样硬化大鼠的红细胞DI（0.35±0.01对0.31±0.02）、血浆NO[（3.9±0.7）nmol/ml对（2.8±0.7）nmol/ml]和T-AOC水平[（8.0±0.6）U/ml对（6.7±0.6）U/ml]（均P < 0.05）。
n-3PUFAs可能改善动脉粥样硬化大鼠的血液流变学和凝血功能，减轻氧化应激，改善内皮功能，抑制动脉粥样硬化斑块形成。
