School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
Biointerphases. 2012 Dec;7(1-4):30. doi: 10.1007/s13758-012-0030-1. Epub 2012 Apr 24.
To enhance the cytocompatibility of polycaprolactone (PCL), cell-adhesive gelatin is covalently immobilized onto the PCL film surface via two surface-modified approaches: a conventional chemical immobilization process and a surface-initiated atom transfer radical polymerization (ATRP) process. Kinetics studies reveal that the polymer chain growth from the PCL film using the ATRP process is formed in a controlled manner, and that the amount of immobilized gelatin increases with an increasing concentration of epoxide groups on the grafted P(GMA) brushes. In vitro cell adhesion and proliferation studies demonstrate that cell affinity and growth are significantly improved by the immobilization of gelatin on PCL film surfaces, and that this improvement is positively correlated to the amount of covalently immobilized gelatin. With the versatility of the ATRP process and tunable grafting efficacy of gelatin, this study offers a suitable methodology for the functionalization of biodegradable polyesters scaffolds to improve cell-material interactions.
为了提高聚己内酯（PCL）的细胞相容性，通过两种表面改性方法将细胞黏附性明胶共价固定在 PCL 薄膜表面：传统的化学固定化过程和表面引发原子转移自由基聚合（ATRP）过程。动力学研究表明，使用 ATRP 过程从 PCL 薄膜上进行的聚合物链增长是以受控的方式形成的，并且固定化明胶的量随着接枝的 P（GMA）刷上环氧基团浓度的增加而增加。体外细胞黏附和增殖研究表明，通过在 PCL 薄膜表面固定化明胶，显著提高了细胞亲和力和生长，并且这种改善与共价固定化明胶的量呈正相关。由于 ATRP 过程的多功能性和明胶接枝效率的可调性，本研究为改善细胞-材料相互作用的可生物降解聚酯支架的功能化提供了一种合适的方法。
