Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
J Nutr. 2010 Dec;140(12):2158-61. doi: 10.3945/jn.110.130302. Epub 2010 Oct 27.
Previously, we showed that supplementation of diets with short-chain inulin (P95), long-chain inulin (HP), and a 50:50 mixture of both (Synergy 1) improved body iron status and altered expression of the genes involved in iron homeostasis and inflammation in young pigs. However, the effects of these 3 types of inulin on intestinal bacteria remain unknown. Applying terminal restriction fragment length polymorphism analysis, we determined the abundances of luminal and adherent bacterial populations from 6 segments of the small and large intestines of pigs (n = 4 for each group) fed an iron-deficient basal diet (BD) or the BD supplemented with 4% of P95, Synergy 1, or HP for 5 wk. Compared with BD, all 3 types of inulin enhanced (P < 0.05) the abundance of beneficial bifidobacteria and lactobacilli in the microbiota adherent to intestinal mucus of various gut segments of pigs. These changes were seen as proximal as in the jejunum with P95 but did not appear until the distal ileum or cecum with HP. Similar effects of inulin on bacterial populations in the lumen contents were found. Meanwhile, all 3 types of inulin suppressed the less desirable bacteria Clostridium spp. and members of the Enterobacteriaceae in the lumen and mucosa of various gut segments. Our findings suggest that the ability of dietary inulin to alter intestinal bacterial populations may partially account for its iron bioavailability-promoting effect and possibly other health benefits.
先前，我们已经证明，在饮食中补充短链菊粉（P95）、长链菊粉（HP）和两者的 50:50 混合物（Synergy 1）可以改善机体铁状态，并改变铁稳态和炎症相关基因的表达年轻猪。然而，这 3 种菊粉对肠道细菌的影响尚不清楚。应用末端限制性片段长度多态性分析，我们确定了缺铁基础日粮（BD）或补充 4%的 P95、Synergy 1 或 HP 的日粮喂养的猪 6 个小肠和大肠段的腔内和黏附细菌群的丰度（每组 4 只）5 周。与 BD 相比，所有 3 种菊粉都增加了（P<0.05）猪肠道黏液黏附的微生物群中有益双歧杆菌和乳杆菌的丰度。这些变化在空肠近端的 P95 中可见，但在回肠或盲肠的 HP 中才出现。在肠内容物的细菌种群中也发现了菊粉的相似作用。同时，所有 3 种菊粉都抑制了腔和黏膜各肠段中不太理想的细菌梭菌属和肠杆菌科成员。我们的发现表明，膳食菊粉改变肠道细菌种群的能力可能部分解释了其促进铁生物利用度的作用，以及可能的其他健康益处。
