Herosimczyk Agnieszka, Lepczyński Adam, Werkowska Martyna, Barszcz Marcin, Taciak Marcin, Tuśnio Anna, Ciechanowicz Andrzej Krzysztof, Kucia Magdalena, Susfał Karolina, Cabała Sandra, Ożgo Małgorzata
Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland.
Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.
Animals (Basel). 2022 Jul 1;12(13):1710. doi: 10.3390/ani12131710.
Prebiotics are known to have many beneficial effects on intestinal health by modulating the gut microbiota composition, thereby affecting epithelial cell proliferation and metabolism. This study had two aims: (1) to identify the protein constituents in the cecal mucosa of 50-day-old healthy (PIC × Penarlan P76) barrows, and (2) to assess the effects of 4% inclusion of dried chicory root in a cereal-based diet on the cecal mucosa proteome changes. Pigs (eight per group) were randomly allotted to the groups and were fed a control diet from the tenth day of life (C) or a diet supplemented with 4% of died chicory root (CR), for 40 days. At the age of 50 days, animals were sacrificed and cecal tissue samples were collected. It was found that feeding a CR diet significantly decreased the expression of 16 cecal mucosa proteins. Among them, fifteen proteins were down-regulated, while only one (KRT20) was shown to be up-regulated when compared to the C group. Dietary supplementation with CR caused down-expression of metabolism-associated proteins including enzymes involved in the process of glycolysis (G6PD, TPI1, ALDH9A1, CKMT1 and AKR1A1) as well as those engaged in transcriptional and translational activity (PRPF19, EEF1G) and several structural proteins (ACTR3, KRT77, CAP1 and actin). From our findings, it is possible to conclude that dietary chicory root at 4% had beneficial effects on the gut health of pigs as indicated by a changed abundance of certain cecal proteins such as KRT20, SERPINB1, HSP27, ANAXA2 and ANAXA4.
已知益生元通过调节肠道微生物群组成对肠道健康有许多有益影响，从而影响上皮细胞增殖和代谢。本研究有两个目的：（1）鉴定50日龄健康（PIC×Penarlan P76）公猪盲肠黏膜中的蛋白质成分，（2）评估在谷类日粮中添加4%干菊苣根对盲肠黏膜蛋白质组变化的影响。将猪（每组8头）随机分配到各组，从出生后第10天开始饲喂对照日粮（C）或添加4%干菊苣根的日粮（CR），持续40天。在50日龄时，处死动物并收集盲肠组织样本。结果发现，饲喂CR日粮显著降低了16种盲肠黏膜蛋白的表达。其中，15种蛋白下调，与C组相比，只有一种（KRT20）上调。日粮中添加CR导致与代谢相关的蛋白表达下调，包括参与糖酵解过程的酶（G6PD、TPI1、ALDH9A1、CKMT1和AKR1A1）以及参与转录和翻译活性的蛋白（PRPF19、EEF1G）和几种结构蛋白（ACTR3、KRT77、CAP1和肌动蛋白）。从我们的研究结果可以得出结论，日粮中4%的菊苣根对猪的肠道健康有有益影响，这表现为某些盲肠蛋白如KRT20、SERPINB1、HSP27、ANAXA2和ANAXA4的丰度发生了变化。
