Department of Respiratory Medicine and Tuberculosis, Faculty of Medicine, P.J. Safarik University and L. Pasteur Teaching Hospital, Kosice, Slovakia.
Med Sci Monit. 2010 Nov;16(11):CR540-4.
Surfactant protein D (SP-D) is a promising blood biomarker in patients with chronic obstructive pulmonary disease (COPD). Nevertheless, circulating levels of SP-D are not related to pulmonary functions. In the present exploratory study, we created a simple index of plasma to bronchoalveolar lavage (BAL) fluid ratio of SP-D (pSP-D/bSP-D), and determined whether this index would relate to the severity of airflow limitation and hence represent a superior biomarker than pSP-D alone.
MATERIAL/METHODS: In 50 ex and current smokers (mean age 57.6±7.8 years, 74% men), SP-D was measured in BAL fluid and plasma samples, and the relationships between spirometric variables and a composite parameter - the pSP-D/bSP-D ratio were determined.
There was a significant inverse correlation between the pSP-D/bSP-D ratio and the severity of airflow obstruction, as measured by FEV1/FVC (p=0.012). In contrast, no relationship was observed between FEV1/FVC and pSP-D alone.
We suggest that integrating both lung and plasma expression of pneumo-proteins may be more useful than plasma expression alone in developing pneumo-proteins as potential biomarkers in COPD.
表面活性蛋白 D（SP-D）是慢性阻塞性肺疾病（COPD）患者有前途的血液生物标志物。然而，循环 SP-D 水平与肺功能无关。在本探索性研究中，我们创建了一个简单的血浆与支气管肺泡灌洗液（BAL）中 SP-D 比值的指数（pSP-D/bSP-D），并确定该指数是否与气流受限的严重程度相关，从而代表比单独 pSP-D 更优越的生物标志物。
材料/方法：在 50 名既往和当前吸烟者（平均年龄 57.6±7.8 岁，74%为男性）中，测量了 BAL 液和血浆样本中的 SP-D，并确定了肺功能变量与复合参数 pSP-D/bSP-D 比值之间的关系。
pSP-D/bSP-D 比值与 FEV1/FVC （p=0.012）测量的气流阻塞严重程度呈显著负相关。相比之下，在 FEV1/FVC 与单独的 pSP-D 之间未观察到相关性。
我们建议，整合肺部和血浆中肺蛋白的表达可能比单独检测血浆中的肺蛋白表达更有用，从而开发 COPD 中潜在的肺蛋白生物标志物。
