Hong S Y, Lee C S
Department of Internal Medicine, Soonchunhyang University Chunan Hospital, Korea.
Korean J Intern Med. 1990 Jul;5(2):87-92. doi: 10.3904/kjim.1990.5.2.87.
A simple method for the quantitative assay of vascular plasminogen activator is described. From 24 chronic renal failure patients who were undergoing surgery for A/V fistular or shunt construction, small pieces of artery (mean 7.8 mg, range 3.0-18.5 mg) and vein (mean 4.5 mg, range 3.0-8.8 mg) were used. The fibrinolytic activity was assayed on a well-controlled fibrin plate by only distilled water immersion and incubation at 37 degrees C. Basal fibrinolytic activity was correlated with venous fibrinolytic activity (r = 0.773, p less than 0.0001) and with arterial fibrinolytic activity (r = 0.55, 0.005 less than p less than 0.01). The increment of fibrinolytic activity by venous occlusion was correlated to venous fibrinolytic activity (r = 0.849, p less than 0.0001) but not to arterial fibrinolytic activity (r = 0.34, 0.1 less than p less than 0.25).
本文描述了一种定量检测血管纤溶酶原激活剂的简单方法。选取24例因动静脉内瘘或分流术而接受手术的慢性肾衰竭患者，取小块动脉组织（平均7.8mg，范围3.0 - 18.5mg）和静脉组织（平均4.5mg，范围3.0 - 8.8mg）。仅通过蒸馏水浸泡并在37℃孵育，在严格控制的纤维蛋白平板上检测纤溶活性。基础纤溶活性与静脉纤溶活性相关（r = 0.773，p < 0.0001），与动脉纤溶活性相关（r = 0.55，0.005 < p < 0.01）。静脉阻塞后纤溶活性的增加与静脉纤溶活性相关（r = 0.849，p < 0.0001），但与动脉纤溶活性无关（r = 0.34，0.1 < p < 0.25）。
