Friedberg B H, Gartner L P
Baltimore College of Dental Surgery, University of Maryland.
J Endod. 1990 Sep;16(9):434-7. doi: 10.1016/S0099-2399(06)81886-8.
White Leghorn chick embryos were used to investigate the embryotoxicity and teratogenicity of 25 and 50% Buckley's formocresol. Embryos were injected at 48 h and sacrificed on the ninth day of incubation. The percentage of mortality of sham and vehicle controls was 4.8 and 17.7%, respectively, whereas eggs injected with 25 and 50% formocresol displayed a 40 and 100% mortality rate. Gross morphological abnormalities, weights, and crown-rump lengths were determined, demonstrating that Buckley's formocresol is embryotoxic and teratogenic in chick embryos. Gross morphological changes were noted to include cranial hematomas, facial abnormalities, eye and beak deformities as well as alterations in feather germ appearance. Histological changes included alterations in the organization of the eyes, and formation of the beak, palate, vasculature, musculature, cartilage, and bone. Moreover, experimental embryos displayed a retardation of development in that they lagged behind their controls by approximately 24 to 36 h.
使用白来航鸡胚胎研究25%和50%巴克利氏甲醛甲酚的胚胎毒性和致畸性。在48小时时对胚胎进行注射，并在孵化的第九天处死。假手术对照组和赋形剂对照组的死亡率分别为4.8%和17.7%，而注射25%和50%甲醛甲酚的鸡蛋死亡率分别为40%和100%。测定了大体形态异常、重量和冠臀长度，表明巴克利氏甲醛甲酚对鸡胚胎具有胚胎毒性和致畸性。观察到的大体形态变化包括颅骨血肿、面部异常、眼睛和喙部畸形以及羽毛芽外观改变。组织学变化包括眼睛组织结构改变以及喙、腭、脉管系统、肌肉组织、软骨和骨骼的形成。此外，实验胚胎表现出发育迟缓，比对照组落后约24至36小时。
