Kay H M, Birss A J, Smalley J W
Department of Clinical Dental Sciences, University of Liverpool, United Kingdom.
Oral Microbiol Immunol. 1990 Oct;5(5):269-74. doi: 10.1111/j.1399-302x.1990.tb00424.x.
The extracellular vesicles (ECV) and extracellular protein (EP) fractions of Bacteroides gingivalis W50 showed haemagglutinating (HA) activity towards sheep erythrocytes. Similar fractions from the nonpathogenic strain W50/BE1 did not haemagglutinate. W50 ECV HA activity was not inhibited by various glycosidase, phospholipase or protease pretreatments, sugars or amino acids, including arginine or lysine. The haemagglutinating activity of ECV was associated only with the extracellular vesicle membrane. The EP and ECV of both strains displayed haemolytic activity. This activity was apparently depressed in the presence of 10 mM dithiothreitol (DTT). All EP and ECV fractions degraded certain structural sheep erythrocyte membrane proteins. The greatest activity was displayed by W50 ECV and W50/BE1 EP and was enhanced by DTT. In the presence of DTT, the ECV of both strains degraded purified human haemoglobin but this activity was greatly reduced in its absence.
牙龈卟啉单胞菌W50的细胞外囊泡（ECV）和细胞外蛋白（EP）组分对绵羊红细胞表现出血凝（HA）活性。非致病菌株W50/BE1的类似组分则不发生血凝。W50 ECV的HA活性不受各种糖苷酶、磷脂酶或蛋白酶预处理、糖类或氨基酸（包括精氨酸或赖氨酸）的抑制。ECV的血凝活性仅与细胞外囊泡膜相关。两种菌株的EP和ECV均表现出溶血活性。在存在10 mM二硫苏糖醇（DTT）的情况下，这种活性明显降低。所有EP和ECV组分均能降解某些结构的绵羊红细胞膜蛋白。W50 ECV和W50/BE1 EP表现出最大活性，且DTT可增强该活性。在存在DTT的情况下，两种菌株的ECV均可降解纯化的人血红蛋白，但在不存在DTT时该活性会大大降低。
