Smalley J W, Birss A J
Department of Clinical Dental Sciences, University of Liverpool, U.K.
FEMS Microbiol Lett. 1990 Aug;58(3):317-20. doi: 10.1111/j.1574-6968.1990.tb13996.x.
Comparison was made of the specific activities of whole extracellular soluble protein (EP) and extracellular vesicle (ECV)-associated trypsin-like protease (TLP) activity from batch cultures of Bacteroides gingivalis W50. Rapid loss of activity occurred when these fractions were maintained at 37 degrees C in the presence of DTT. Residual levels of activity were detected after incubation of ECV and EP for up to 8 days under non-reducing conditions. Rates of activity loss in EP and ECV were similar. Mixtures of EP and ECV, in the same proportions as found in the culture supernatant showed neither depression nor elevation of total activity from the expected compound activities of the two separate fractions.
对牙龈卟啉单胞菌W50分批培养物中的全细胞外可溶性蛋白（EP）和细胞外囊泡（ECV）相关的类胰蛋白酶（TLP）活性的比活性进行了比较。当这些组分在DTT存在下于37℃保存时，活性迅速丧失。在非还原条件下将ECV和EP孵育长达8天后，检测到了残留的活性水平。EP和ECV中的活性丧失速率相似。EP和ECV的混合物，其比例与培养上清液中发现的相同，总活性既没有低于也没有高于两个单独组分预期的复合活性。
