Smalley J W, Shuttleworth C A, Birss A J
Department of Dental Sciences, University of Liverpool, England.
Arch Oral Biol. 1989;34(7):579-83. doi: 10.1016/0003-9969(89)90098-8.
The activities of the extracellular vesicle fractions of these two organisms were compared. Lytic activity against a native type I placental collagen substrate at 30 degrees C was assessed following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and densitometry. A rapid rate of collagen depolymerization was achieved by the extracellular vesicle fraction of W50, yielding approx. 90% substrate degradation compared to 5% for W50/BE1 extracellular vesicles over 6 h incubation. The polypeptide digestion patterns produced by incubation with extracellular vesicle fractions of both organisms were identical, and similar to those yielded by incubation of substrate with whole W50 cells.
比较了这两种生物体细胞外囊泡组分的活性。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和光密度测定后，评估了在30℃下针对天然I型胎盘胶原底物的裂解活性。W50的细胞外囊泡组分实现了快速的胶原解聚，在6小时的孵育中，与W50/BE1细胞外囊泡的5%相比，底物降解约90%。两种生物体的细胞外囊泡组分孵育产生的多肽消化模式相同，并且与底物与整个W50细胞孵育产生的模式相似。
