Fujimura S, Nakamura T
Matsumoto Dental College, Hiro'oka, Shiojiri, Japan.
Oral Microbiol Immunol. 1990 Dec;5(6):360-2. doi: 10.1111/j.1399-302x.1990.tb00441.x.
From the culture supernatant of Bacteroides gingivalis ATCC 33277, a thiol protease was purified to homogeneity by fractional ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and isoelectric focusing. Its molecular weight was 43 kDa and showed similar enzymatic properties to a 300-kDa protease that was previously characterized.
