组织样品和蛋白质提取物的解冻、复冻和储存条件对 2-DE 斑点强度的影响。

Effects of thawing, refreezing and storage conditions of tissue samples and protein extracts on 2-DE spot intensity.

机构信息

Department of Radiology and Nuclear Medicine (Radiochemistry), Charité Universitätsmedizin, Campus Benjamin Franklin, Berlin, Germany.

出版信息

Proteomics. 2010 Apr;10(7):1515-21. doi: 10.1002/pmic.200900471.

DOI:10.1002/pmic.200900471

PMID:20127687

Abstract

We report that reliable quantitative proteome analyses can be performed with tissue samples stored at -80 degrees C for up to 10 years. However, storing protein extracts at 4 degrees C for 24 h and freezing protein extracts at -80 degrees C and thawing them significantly altered 41.6 and 17.5% of all spot intensities on 2-DE gels, respectively. Fortunately, these storing effects did not impair the reliability of quantifying 2-DE experiments. Nonetheless, the results show that freezing and storage conditions should be carefully controlled in proteomic experiments.

摘要

我们报告称，组织样本在-80°C 下储存长达 10 年，仍可进行可靠的定量蛋白质组分析。然而，将蛋白质提取物在 4°C 下储存 24 小时，以及将蛋白质提取物在-80°C 下冷冻和解冻，分别显著改变了 2-DE 凝胶上所有斑点强度的 41.6%和 17.5%。幸运的是，这些储存效应并没有损害定量 2-DE 实验的可靠性。尽管如此，结果表明在蛋白质组学实验中，应仔细控制冷冻和储存条件。