Matheus M T, Hetem S
Faculdade de Odontologia-UNESP.
Rev Odontol UNESP. 1990;19(1):51-61.
Mice foetus were killed between 13 and 16 days of the gestational period and their molar tooth germs grafted into the anterior chamber of the eye. The hosts were injected either with 6.25 mg/Kg or with 125 mg/Kg of cyclophosphamide and killed 15 days later. The cyclophosphamide irreversibly, interferes on tooth germ development causing either its total degeneration or an alteration on its morphology forming hypodeveloped tooth germs; its action is directly proportional to the foetus age and to the dose used; it does not induce the formation of osteodentin while some predentin partial cell inclusion may occur.
在妊娠期13至16天之间处死小鼠胎儿，并将其磨牙牙胚移植到眼前房。给宿主注射6.25毫克/千克或125毫克/千克的环磷酰胺，15天后处死。环磷酰胺不可逆转地干扰牙胚发育，导致其完全退化或形态改变，形成发育不全的牙胚；其作用与胎儿年龄和所用剂量成正比；它不会诱导骨样牙本质的形成，但可能会出现一些前期牙本质部分细胞包埋的情况。
