Cooper A D, Jefferies T M
School of Pharmacy and Pharmacology, University of Bath, UK.
J Pharm Biomed Anal. 1990;8(8-12):847-51. doi: 10.1016/0731-7085(90)80131-8.
A procedure is described which allows the on-line recovery of enantiomers following semi-preparative chiral separation by RP-HPLC using beta-cyclodextrin in the mobile phase. By this method, the phenothiazine antihistamine trimeprazine (I) was resolved into its antipodes at greater than 95% optical purity at a throughput of more than 1 mg of each enantiomer per hour, using 4 mm i.d. columns. The recovered trimeprazine was found to be free from cyclodextrin.
本文描述了一种方法，该方法可在使用β-环糊精作为流动相通过反相高效液相色谱（RP-HPLC）进行半制备手性分离后在线回收对映体。通过该方法，使用内径为4 mm的色谱柱，吩噻嗪类抗组胺药曲美苄嗪（I）以大于95%的光学纯度拆分为其对映体，每小时每种对映体的通量超过1 mg。回收得到的曲美苄嗪未发现含有环糊精。
