垂体“脂肪代谢激素”的研究。VIII. 脂肪分解因子P-LF II D的放射免疫测定

Studies on the pituitary "Fettstoffwechselhormon". VIII. Radioimmunoassay of the lipolytic factor P-LF II D.

作者信息

Etzrodt H, Schleyer M, Pfeiffer E F

出版信息

Horm Metab Res. 1978 Jul;10(4):299-304. doi: 10.1055/s-0028-1093419.

Abstract

A radioimmunoassay of the lipolytic peptide P-LF II D from porcine pituitaries is described. The assay is performed with 125-iodine labeled P-LF II D and with antisera either from guinea pigs or from rabbits. Bound antigen is separated from the free by double antibody technique. No cross reaction is observed with gamma lipotropin, peptide B, secretin, glucagon, isoproterenol. Due to contamination P-LF II C, beta lipotropin and human growth hormone displace the tracer when added at large doses. Complete cross reaction is observed between porcine 1-39 ACTH and P-LF II D. Specificity of this reaction is demonstrated by the increase of cross reaction, when ACTH fragments of increasing length of the polypeptide chain are used (1-23 ACTH, 1-24 ACTH and 1-28 ACTH).

摘要

本文描述了一种对猪垂体中脂解肽P-LF II D的放射免疫分析方法。该分析采用125碘标记的P-LF II D以及豚鼠或兔的抗血清进行。通过双抗体技术将结合抗原与游离抗原分离。未观察到与γ促脂素、肽B、促胰液素、胰高血糖素、异丙肾上腺素的交叉反应。由于污染，大剂量添加P-LF II C、β促脂素和人生长激素时会使示踪剂移位。猪1-39促肾上腺皮质激素（ACTH）与P-LF II D之间观察到完全交叉反应。当使用多肽链长度不断增加的ACTH片段（1-23 ACTH、1-24 ACTH和1-28 ACTH）时，交叉反应增加，证明了该反应的特异性。