Majchrzak D, Gronowska-Senger A
Zakładu Oceny Zywienia SGGW-AR w Warszawie.
Rocz Panstw Zakl Hig. 1990;41(5-6):239-45.
The limit levels of potassium nitrate and sodium nitrite were determined in the diet of rats receiving orally beta-carotene. Two 30-day biological experiments were done on male Wistar rats standardized for vitamin A level in the liver. The animals were given semisynthetic diet, without vitamin A, containing definite amounts of potassium nitrate or sodium nitrite: 4.0; 4.5; 5.0; 5.5; 6.0% of the nitrate, and 0.5; 0.6; 0.7; 0.8; 0.9; 1.0% of the nitrite. The rats were given also orally two drops of an oil solution of beta-carotene in doses of 30 micrograms per rat daily. The limit of tolerance was established measuring beta-carotene utilization according to the method of balance studies and liver function test. Diet consumption and body weight of the animals were considered as well. It was found that in this experiment the limit dose, that is the dose of nitrates and nitrites over which the rats died, was 5.0% for nitrate and 0.7% for nitrite.
测定了经口给予β-胡萝卜素的大鼠饮食中硝酸钾和亚硝酸钠的限量水平。对肝脏中维生素A水平标准化的雄性Wistar大鼠进行了两项为期30天的生物学实验。给动物喂食不含维生素A的半合成饮食，其中含有一定量的硝酸钾或亚硝酸钠：硝酸盐含量分别为4.0%、4.5%、5.0%、5.5%、6.0%，亚硝酸盐含量分别为0.5%、0.6%、0.7%、0.8%、0.9%、1.0%。大鼠每天还经口给予两滴β-胡萝卜素油溶液，每只大鼠剂量为30微克。根据平衡研究方法和肝功能测试来测定β-胡萝卜素的利用率，从而确定耐受限量。同时也考虑了动物的饮食消耗量和体重。结果发现，在本实验中，大鼠死亡时的硝酸盐和亚硝酸盐限量剂量，硝酸盐为5.0%，亚硝酸盐为0.7%。
