Isom H, Colberg A, Reed C, Rapp F
Int J Cancer. 1978 Jul 15;22(1):22-7. doi: 10.1002/ijc.2910220106.
Mouse cells (line N cIA cl10) contain 1.2-2.5 ng murine leukaemia virus (MuLV) p30 antigen/mg of protein; this amount of antigen is measurable by competition radioimmunoassay (RIA) but is not detectable by indirect immunofluorescence (IF). Infection of N cIA cl10 cells with herpes simplex virus type 2 (HSV-2) induces expression of MuLV p30. Induction by HSV-2 does not require either cell or virus DNA synthesis and is optimal 8 h post infection when cells at 50-70% confluence are infected at a multiplicity of infection (MOI) of 5-8 PFU/cell. At an MOI of 2.5, 70-80% of the cells express HSV antigens while none of the cells express p30; at an MOI of 5.0, 70-80% of the cells express HSV antigens but 55% of the cells express p30. Using the conditions reported in this paper for preparation of competing antigen, induction of p30 by HSV-2 (strain 333) infection is not measurable by competition RIA.
小鼠细胞（N cIA cl10系）每毫克蛋白质含有1.2 - 2.5纳克鼠白血病病毒（MuLV）p30抗原；该抗原量可用竞争放射免疫测定法（RIA）检测到，但用间接免疫荧光法（IF）检测不到。用2型单纯疱疹病毒（HSV - 2）感染N cIA cl10细胞可诱导MuLV p30的表达。HSV - 2诱导不需要细胞或病毒DNA合成，当处于50 - 70%汇合度的细胞以5 - 8个空斑形成单位（PFU）/细胞的感染复数（MOI）进行感染时，感染后8小时诱导效果最佳。在MOI为2.5时，70 - 80%的细胞表达HSV抗原，但没有细胞表达p30；在MOI为5.0时，70 - 80%的细胞表达HSV抗原，但55%的细胞表达p30。按照本文报道的制备竞争抗原的条件，HSV - 2（333株）感染诱导的p30用竞争RIA检测不到。
